Identification of a Novel Anticancer Oligopeptide from Perilla frutescens (L.) Britt. and Its Enhanced Anticancer Effect by Targeted Nanoparticles In Vitro

Figure 1

Purification of oligopeptides and their cytotoxicity screening. (a) Gel filtration chromatography on a Sephadex G-25 column, and there were three peaks, PSO_(G25)1, PSO_(G25)2, and PSO_(G25)3. (b) Anticancer effect of three fractions on U251, A549, HCT116, HepG2, and MGC-803 with 1 mg/ml or 5 mg/ml for 24 h, measured by the MTT assay. Percentages of cell proliferation were normalized by optical density values of the vehicle group (as 100%). Data are shown as means ± SD (). denotes , denotes , and denotes ; comparisons were performed between groups of 1 mg/ml and 5 mg/ml of every fraction. # denotes , ## denotes , and ### denotes ; comparisons were performed against the vehicle group.

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