Analytical Methods to Characterize and Purify Polymeric Nanoparticles

Figure 4

Example of an EMSA of polymeric nanoparticles conjugated with antisense oligonucleotides at different nanoparticle/oligonucleotide (N/P) charge ratios. When the complexation is achieved (zero surface charge), the band is diffused (at 0.75/1 in this case).