Analytical Methods to Characterize and Purify Polymeric Nanoparticles

Table 4

Summary of the main advantages and disadvantages of the described purification techniques.

Technique	Use	Advantages	Disadvantages	References
Filtration	Purification	Useful for thermolabile compounds	Time consuming	Scopes [44]
	Sterilization
	Concentration	Rapid and simple	Bigger sizes determined by the cut-off size	Sapsford et al. [9]
	Dispersant changement	Commercially available devices	Single-use devices	Roy et al. [43]
	Reduce size polydispersity	Cost effective		Dobrovolskaia and McNeil [42]
Centrifugation	Purification	High efficiency	Special equipment required for large volumes	Scopes [44]
	Concentration	Rapid, facile, and economic	Difficult to resuspend soft matter	Sapsford et al. [9]
	Dispersant changement	Low amounts of sample
		Appropriate for different kinds of nanomaterials
Dialysis	Purification	Rapid, facile, and economic	Limited to the membranes MWCO	Vauthier et al. [46]
	Concentration	Commercially available devices	High receptor solution volumes	Sapsford et al. [9]
	Dispersant changement	No sample pretreatment
Electrophoresis	Purification	Simple and economic	Postelectrophoresis purification steps	Sapsford et al. [9]
				Jason [51]
		High resolution and sensitivity
			Need of charged compounds	Scopes [45]