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International Journal of Polymer Science
Volume 2019, Article ID 7068437, 7 pages
https://doi.org/10.1155/2019/7068437
Research Article

Effect of Codonopsis pilosula Polysaccharides on the Growth and Motility of Hepatocellular Carcinoma HepG2 Cells by Regulating β-Catenin/TCF4 Pathway

1Department of Laboratory Medicine, The Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University, Huai’an, Jiangsu 223300, China
2Department of Cardiology, The Affiliated Huaian No. 1 People's Hospital of Nanjing Medical University, Huai’an, Jiangsu 223300, China

Correspondence should be addressed to Yuan-yuan Zhang; moc.361@4321gnahznauynauy

Received 11 March 2019; Revised 12 April 2019; Accepted 15 April 2019; Published 8 May 2019

Guest Editor: Jianxun Ding

Copyright © 2019 Yuan-yuan Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. To study the effect of Codonopsis pilosula polysaccharide (CPP) on the growth and motility of HepG2 cells and its possible mechanism. Methods. Cells were randomly divided into Control group, CPP (5 μM) group, CPP (10 μM) group, and CPP (20 μM) group. The proliferation, invasion, migration ability, and expression of proteins involved in the epithelial-mesenchymal transition (EMT) and signaling pathway of HepG2 cells were detected by CCK8 assay, BrdU staining, Transwell, Scratch test, and Western blot, respectively. Results. Codonopsis pilosula polysaccharide inhibited the proliferation of HepG2 cells cultured in vitro along with the expression level of Ki67 and PCNA protein (), decreased the number of invasive cells (), and reduced the scratch closure rate (). It also adjusted the expression of vascular endothelial growth factor (VEGF), E-cadherin, and N-cadherin (). Other than that, downregulation of β-catenin, TCF4, and c-Myc protein expression () was observed as well. Conclusion. Codonopsis pilosula polysaccharide can inhibit the proliferation and motility of HepG2 cells cultured in vitro, and the underlying mechanism is proposed to be related to the inhibition of the β-catenin/TCF4 pathway.