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International Journal of Rheumatology
Volume 2012, Article ID 724872, 6 pages
http://dx.doi.org/10.1155/2012/724872
Clinical Study

The Association of IL-12b Polymorphisms with Systemic Lupus Erythematosus in Chinese Han Population

Yong Shao,1,2 Jie Zhang,1,2 Yuewen Chen,1,3 Qi Wu,1,3 Ming Guan,4 Bo Yu,2 Jun Wan,1,3,5 and Wei Zhang1,3,5

1Shenzhen Key Lab for Translational Medicine of Dermatology, Shenzhen PKU-HKUST Medical Center, No. 1120, Lianhua Road, Futian District, Shenzhen, Guangdong 518036, China
2Department of Dermatology, Shenzhen Hospital Peking University, Shenzhen, Guangdong 518036, China
3Biomedical Research Institute, Shenzhen PKU-HKUST Medical Center, No. 1120, Lian-Hua Road, Fu-Tian District, Shenzhen, Guangdong 510632, China
4Department of Clinical Laboratory, Huashan Hospital, Shanghai 200040, China
5Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong

Received 24 January 2012; Accepted 16 March 2012

Academic Editor: Javier Martin

Copyright © 2012 Yong Shao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Systemic lupus erythematosus (SLE) is a complex immune disease. The genetic variation in the IL-12b gene was found to associate with SLE in Caucasian population. In this study, we examined this association in Chinese Han population by a recently developed method, unlabeled probe-based high resolution melting analysis. Methods. A total of 297 SLE patients and 351 controls were recruited. Unlabeled probe-based high resolution melting analysis (HRMA) was used in genotyping. Results. Statistically significant differences were observed in both genotype and allele frequencies for rs6887695 in the SLE patients as compared with the controls. Minor allele (C) of rs6887695 ( , OR 0.78, [95% CI 0.63-0.98]) was found to be protective against SLE. The association of SNP rs6887695 with the diagnostic criteria of SLE was also examined. Minor allele (C) exerts protective effect on the incidence of arthritis ( , OR = 0.65, 95% CI = 0.47-0.92) and abnormalities of antinuclear antibody ( , OR = 0.68, 95% CI = 0.49–0.95). IL-12b SNPs were irrelevant to other diagnostic criteria of SLE. Summary. Polymorphisms of rs6887695 in IL-12b gene were associated with disease risk, as well as arthritis and antinuclear antibody synthesis, of systemic lupus erythematosus in Chinese population.