Figure 2: Detection of Foxp3-positive cells in the pancreas. Single immunostaining of CD4 (green; FITC) and Foxp3 (red; Cy3) in poly I:C-treated MRL/Mp mice at the age of 18 weeks. Nuclei were stained by DAPI (blue nuclear stain). The merged images demonstrated many mononuclear cells double positive for CD4 and Foxp3 in the pancreas. The most Foxp3-positive cells appeared in the CD4-positive cells (×400).