Immune Mediators in Osteoarthritis: Infrapatellar Fat Pad-Infiltrating CD8+ T Cells Are Increased in Osteoarthritic Patients with Higher Clinical Radiographic Grading

Figure 1

T cell frequency in peripheral blood, synovial tissue, and infrapatellar fat pad of knee osteoarthritis patients. Mononuclear cells were isolated from peripheral blood, synovial tissue, and infrapatellar fat pad. Cells were phenotypically characterized based on CD3+, CD4+, and CD8+ surface expression. (a) Graph comparing CD3+ T cell frequency of total mononuclear cells in peripheral blood (), synovial tissue (), and infrapatellar fat pad (). (b) Graphs showing CD3+ T cell frequency of total mononuclear cells in synovial tissue and infrapatellar fat pad stratified based on Ahlback clinical grading (grades 1–4). Synovial tissue grades I (), II (), III (), and IV (); infrapatellar fat pad grades I (), II (), III (), and IV (). (c) Bar graphs showing composition of different T cell subset (CD4+, CD8+, and DN T cells) frequencies isolated from peripheral blood (PBMC) (), synovial tissue (), and infrapatellar fat pad (). Bars are mean values with error bars.

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