Integration of Genome-Wide DNA Methylation and Transcription Uncovered Aberrant Methylation-Regulated Genes and Pathways in the Peripheral Blood Mononuclear Cells of Systemic Sclerosis

<div><b>QPCR validation of gene expression: 20 MeDEGs were validated by QPCR in a separate cohort of SSc (n=12) and NC (n=12) samples.</b> 11 MeDEGs (7 upregulated and 4 downregulated) were confirmed to be differentially expressed between SSc and NC (<i>p</i>&lt;0.05) by QPCR and 6 MeDEGs (3 upregulated and 3 downregulated) showed the difference in expression but did not reached statistical significance. Inconsistency in the detection of the expression for three MeDEGs by microarray or QPCR was also noted.</div>

International Journal of Rheumatology

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Figure 4: Integration of Genome-Wide DNA Methylation and Transcription Uncovered Aberrant Methylation-Regulated Genes and Pathways in the Peripheral Blood Mononuclear Cells of Systemic Sclerosis 

Figure 4 | Integration of Genome-Wide DNA Methylation and Transcription Uncovered Aberrant Methylation-Regulated Genes and Pathways in the Peripheral Blood Mononuclear Cells of Systemic Sclerosis 