Figure 4: QPCR validation of gene expression: 20 MeDEGs were validated by QPCR in a separate cohort of SSc (n=12) and NC (n=12) samples. 11 MeDEGs (7 upregulated and 4 downregulated) were confirmed to be differentially expressed between SSc and NC (p<0.05) by QPCR and 6 MeDEGs (3 upregulated and 3 downregulated) showed the difference in expression but did not reached statistical significance. Inconsistency in the detection of the expression for three MeDEGs by microarray or QPCR was also noted.