Selenium Attenuates HPV-18 Associated Apoptosis in Embryo-Derived Trophoblastic Cells but Not Inner Cell Mass In Vitro

Figure 1

Implanted mouse trophoblasts cells after 48-hour exposure (at 37°C, 5% CO₂ in air) to either (1) control medium with heat-inactivated HPV-18 HeLa lysate, (2) HPV-18 HeLa lysate, (3) HPV-18 HeLa lysate and 0.5 μM selenomethionine (SeMet), or (4) HPV-18 HeLa lysate and 5.0 μM SeMet. The stain used was Rose Bengal and Fast Green-based Spermac staining kit and images were taken using the Nikon Diaphot inverted microscope (400x magnification). The cell sizes for the images were as follows: (1) control, 515 μ, (2) HPV-18 HeLa lysate, 609 μ, (3) HPV-18 HeLa lysate with 0.5 μM SeMet, 622 μ, and (4) HPV-18 HeLa lysate with 5.0 μM SeMet, 915 μ at the greatest width.