Selenium Attenuates HPV-18 Associated Apoptosis in Embryo-Derived Trophoblastic Cells but Not Inner Cell Mass In Vitro

Table 1

Comparison of the area (mean ± S.E.M.) of each trophoblast nucleus or each trophoblast cell size after 24-hour exposure (at 37°C, 5% CO₂ in air) to either (1) control medium with heat-inactivated HPV-18 HeLa lysate, (2) HPV-18 HeLa lysate, (3) HPV-18 HeLa lysate and 0.5 µM selenomethionine (SeMet), or (4) HPV-18 HeLa lysate and 5.0 µM SeMet. The ICM cells were not analyzed due to limitations in stained compacted cells.

Treatment groups	Number of cells ()	Size of nucleus (µ^2)	Size of trophoblast (µ^2)
(1) Control	23	355.6 ± 35.9	45,620.5 ± 10,440.3
(2) HPV-18 HeLa lysate	28	253.8 ± 28.5^a	54,477.3 ± 10,352.0
(3) HPV-18 HeLa lysate and 0.5 µM SeMet	20	401.9 ± 47.8^b	63,651.1 ± 16,106.9^a
(4) HPV-18 HeLa lysate and 5.0 µM SeMet	26	275.5 ± 23.1	105,038.0 ± 8,802.0^a,b

Different from the control (1) group ().
^bDifferent from the HPV-18 HeLa lysate (2) group ().