Selenium Attenuates HPV-18 Associated Apoptosis in Embryo-Derived Trophoblastic Cells but Not Inner Cell Mass In Vitro

Table 2

Day 6 in vitro implanted mouse embryos were exposed (at 37°C, 5% CO₂ in air) for 48 hours to either (1) control medium with heat-inactivated HPV-18 HeLa lysate, (2) HPV-18 HeLa lysate, (3) HPV-18 HeLa lysate and 0.5 µM selenomethionine (SeMet), or (4) HPV-18 HeLa lysate and 5.0 µM SeMet. The percentages of live, apoptotic, and necrotic trophoblast cells were assessed using bisbenzimide and propidium iodide dual-stain epifluorescence analyses.

Trophoblast cells group	Total cells ()	Number of viable cells (%)	Number of apoptotic cells (%)	Number of necrotic cells (%)	Number of total nonviable cells (%)
(1) Control group	64	17 (23.6)	47 (76.4)	0 (0)	47 (76.4)
(2) HPV-18 HeLa lysate	134	16 (13.3)^a	109 (79.8)	9 (6.9)^a	118 (86.7)^a
(3) HPV-18 HeLa and 0.5 µM SeMet	187	51 (24.2)^b	111 (62.2)^b	25 (13.6)^a,b	136 (75.8)^b
(4) HPV-18 HeLa lysate and 5.0 µM SeMet	132	35 (24.0)^b	74 (58.7)^b	23 (17.4)^a,b	97 (76.1)^a,b

Different from the control (1) group ().
^bDifferent from the HPV-18 HeLa lysate (2) group ().