Selenium Attenuates HPV-18 Associated Apoptosis in Embryo-Derived Trophoblastic Cells but Not Inner Cell Mass In Vitro

Table 3

Day 6 in vitro implanted mouse embryos were exposed (at 37°C, 5% CO₂ in air) for 48 hours to either (1) control medium with heat-inactivated HPV-18 HeLa lysate, (2) HPV-18 HeLa lysate, (3) HPV-18 HeLa lysate and 0.5 µM selenomethionine (SeMet), or (4) HPV-18 HeLa lysate and 5.0 µM SeMet. The percentages of live, apoptotic, and necrotic inner cell mass (ICM) cells were assessed using bisbenzimide and propidium iodide dual-stain epifluorescence analyses.

Treated ICM cells groups	Total cells ()	Number of viable cells (%)	Number of apoptotic cells (%)	Number of necrotic cells (%)	Number of total nonviable cells (%)
(1) Control group	71	32 (45.5)	38 (53.2)	1 (1.3)	39 (54.5)
(2) HPV-18 HeLa lysate	365	41 (17.5)^a	236 (55.3)	88 (27.2)^a	324 (82.5)^a
(3) HPV-18 HeLa and 0.5 µM SeMet	236	44 (19.2)^a	123 (50.6)	69 (30.1)^a	192 (80.8)^a
(4) HPV-18 HeLa lysate and 5.0 µM SeMet	210	36 (20.6)^a	148 (68.0)	26 (11.4)^a,b	174 (79.4)^a

Different from the control (1) group ().
^bDifferent from the HPV-18 HeLa lysate (2) group ().