Phospholipase A2 Mediates Apolipoprotein-Independent Uptake of Chylomicron Remnant-Like Particles by Human Macrophages
Figure 5
Role of macrophage-secreted lipases on [3H]TG-CRLPw/o internalization. Cell radioactivity associated with macrophage lipids was determined after a 6-h incubation of HMDM with 80 μg cholesterol/mL of [3H]TG-CRLPw/o in the presence of 10 and 30 μM way121.989 (way), 0.2 and 2 μM of manoalide, 0.2 and 2 μM of orlistat or in the absence of any inhibitor (control). Radioactivity associated with free fatty acids and cholesteryl ester was negligible, and that associated with macrophage triacylglycerol ([3H]TG, (a)), phospholipid ([3H]PL, (b)), and [3H](TG + PL) (c) is reported as nmol/h/mg protein (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 versus control; #P < 0.05, ##P < 0.01 versus manoalide 2 μM.
