Correlations between Endothelial Functions and ROS Detection in Diabetic Microvascular Wall: Early and Late Ascorbic Acid Supplementation
Figure 1
Experimental protocol for measuring ROS content and vascular response to Ach (acetylcholine) as the effect of AA (ascorbic acid) and/or 6R-BH4 (6R-5,6,7,8-tetrahydro-L-biopterin dihydrochloride) application after inhibiting BH4 biosynthetic by DAHP (2,4-diamino-6-hydroxypyrimidine). There are four groups: control (CON + vehicle), control treated with DAHP (CON + DAHP), control treated with DAHP with ascorbic acid (CON + DAHP + AA), and control treated with DAHP with ascorbic acid and BH4 (CON + DAHP + AA + BH4).
