Correlations between Endothelial Functions and ROS Detection in Diabetic Microvascular Wall: Early and Late Ascorbic Acid Supplementation

Table 3

Percentage changes of ROS (intensities/100-μm arteriolar length) and arteriolar diameters in response to Ach from mesenteric arterioles in the presence or absence of BH₄ synthetic inhibitor (DAHP, 20 mM), DAHP plus AA, and DAHP plus BH₄ donor (0.1 mM) in combination of vitamin C in control (CON) rat group.

Group	% changes of ROS; (intensities/100-μm arteriolar length)	% changes of arteriolar diameters
	6-wks CON
CON + Vehicle	54.48±14.90; (𝑛=4)	13.22±1.24; (𝑛=8)
CON + DAHP	169.61±6.46 ***; (𝑛=4)	4.34±0.78 ***; (𝑛=7)
CON + DAHP + AA	115.66±10.96^aaa; (𝑛=3)	17.98±1.42^aaa; (𝑛=6)
CON + DAHP + BH4 0.1 mM + Vit. C	33.89±7.62^aaa; (𝑛=3)	22.10±1.41^aaa; (𝑛=6)

Values are mean ± SEM.
^∗∗∗𝑃<0.001, significantly difference compared to 6-wk vehicle.
^a𝑃<0.05,  ^aaa𝑃<0.001, significantly difference compared to 6-wk DAHP.