Myosin VI and Associated Proteins Are Expressed in Human Macrophages but Do Not Play a Role in Foam Cell Formation in THP-1 Cells

Figure 6

Relative distributions of Myo6 and F-actin in THP-1 macrophages and HMDM. THP-1 macrophages (a) or HMDM (b) were fixed and probed using double immunofluorescence for Myo6 (secondary antibody conjugated to Alexa488 (green)) and F-actin (visualised using rhodamine-conjugated phalloidin (red)); Z-stacked images were taken on a Zeiss LSM or Leica SP5 confocal microscope. Maximum projections are shown on the left for orientation. (a) (i) shows a confocal slice at the level of the bottom of the cells, taken from the region indicated to the left. The individual channels for F-actin (red), Myo6 (green), and the overlay are shown side-by-side. (a) (ii) and (b) (i) show single confocal sections and vertical xz and yz sections through the centre of the cells indicated to the left. The individual channels for F-actin (red), Myo6 (green), and the overlay are shown side-by-side. Typical images from at least 3 separate experiments with of THP-1 cells or HMDM from 3 individual donors are shown. Scale bars = 10 μm.