Etanercept Suppresses Arteritis in a Murine Model of Kawasaki Disease: A Comparative Study Involving Different Biological Agents
Induction of vasculitis by CAWS administration. Arterial inflammation around the aortic root in control mice (a, b) and the CAWS group (no treatment) at 2 weeks (c, d) and at 4 weeks (e, f). (a) and (b): no significant histological abnormalities are evident in the aortic root and coronary arteries in the negative control group. (c) and (d): at 2 weeks, the active inflammatory infiltrates, composed of numerous neutrophils and lymphocytes, segmentally or partially involved the aortic root ((c), arrowheads). The elastic lamillae are focally disrupted ((d), arrowheads). Exudation of fibrin was seen in the subendothelial region (arrow). (e) and (f): at 4 weeks, the inflammation circumferentially involved the aortic root extending from the adventitia throughout the surrounding connective tissue and heart ventricles ((e), arrowheads). The disruption of the vascular walls due to loss of internal and external elastic lamina was more extensive compared to that at 2 weeks ((f), arrowheads). Chronic changes, represented by accumulation of collagen fibers, progressed. Hematoxylin and eosin (H&E) stain ((a), (c), (e)), Elastica-Masson Goldner (EMG) stain ((b), (d), (f)). (a)–(d) Image at original magnification of 100; (e) and (f) images at original magnification of 40. Scale bars: 500 μm (a)–(d) and 1 mm (e, f).