Inhibition of Angiogenesis In Vitro by Chebulagic Acid: A COX-LOX Dual Inhibitor
Figure 2
Effect of CA on the expression of CD31 and E-selectin. HUVECs were maintained in culture in MCDB 131 medium containing 10% FBS supplemented with 25 μM CA for 48 hours. The levels of cell-associated CD31 (a) and E-selectin secreted into the medium (b) at 24 and 48 hours were estimated by ELISA using anti-CD31 (1 : 1000) and anti-E-selectin (1 : 1000) antibody, respectively. Values given are the average of duplicate analysis of 5 experiments ± SEM ().
