Dependence of Proximal GC Boxes and Binding Transcription Factors in the Regulation of Basal and Valproic Acid-Induced Expression of t-PA

Figure 2

Basal signal levels of t-PA reporter construct and its dependence on proximal GC boxes. HT-1080 (a) and HUVEC (b) cells were cotransfected with a firefly luciferase construct containing different variants of a 318 bp insert of the t-PA proximal promoter and a SV40 driven pGL4.73 renilla luciferase control vector. Firefly luciferase signals were normalized to renilla luciferase. The figures show relative basal signal levels in unstimulated reporter constructs and mean values of three (HT-1080) and four (HUVEC) independent experiments. The variants of the t-PA insert were unmutated (intact), CRE mutated, GC box II mutated, GC box III mutated, and no insert (empty pGL4.10 vector). Statistical evaluation was performed using paired Student’s -test and is made relative to constructs with intact insert. , , and .

(a)

(b)