Dependence of Proximal GC Boxes and Binding Transcription Factors in the Regulation of Basal and Valproic Acid-Induced Expression of t-PA

Figure 7

t-PA mRNA expression after siRNA mediated knock-down of p300 and PCAF. HUVECs were treated with p300 or PCAF-targeting siRNAs for 72 h and then stimulated with 3 mM VPA for 24 h. Statistical comparisons are made relative to untreated control cells. . The results are presented as mean values ± SEM of three independent experiments. The statistical evaluation was performed using paired Student’s -test together with Bonferroni correction for multiple analysis. Not shown in the illustrations are transfection controls with transfection reagent only (mock) and transfection with “All Star Negative” control siRNA (ASN) that were included in each experiment. These negative controls showed no significant effect on either t-PA or target mRNA.