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International Journal of Zoology
Volume 2012, Article ID 352165, 7 pages
http://dx.doi.org/10.1155/2012/352165
Research Article

Genetic Characterization of Six Stocks of Litopenaeus vannamei Used in Cuba for Aquaculture by Means of Microsatellite Loci

1Conservation Genetic Group, Marine Research Centre, University of Havana, Street 16 No 114, Playa Havana, CP 11300, Cuba
2Molecular Biology Laboratory, Aquaculture Division, Fisheries Research Centre, 5th Avenue and 246, Barlovento, Playa, Havana, CP 19100, Cuba
3Department of Biochemistry, Faculty of Biology, Havana University, Street 25 No. 455 between J. and I. Vedado, Havana, Cuba

Received 30 August 2011; Revised 1 November 2011; Accepted 11 November 2011

Academic Editor: Pung-Pung Hwang

Copyright © 2012 Anna Pérez-Beloborodova et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Four microsatellite loci were used to achieve genetic characterization of six stocks from Litopenaeus vannamei used for aquaculture in Cuba: second generation from first introduction (S2-1), first generation from the second one (S1-2), from the third one (S1-3), and the fourth one (S1-4) and the crossings from two parental population: first generation from the first with first generation from the third (S1-1 × S1-3) and first generation from the second with first generation from the third (S1-2 × S1-3). 66% (16/24) of genetic systems in total loci were in genetic disequilibrium. The four microsatellite loci were polymorphic for all six stocks. Major quantities of allelic variants correspond to locus Pvan 1758, which is at the same time that one where there are private alleles from first generation of the third. All Fst comparisons were significant. This indicates big differences between stocks. The highest values are those in which there is presence of the second introduction. This introduction and its descendants are also more consanguineous.