International Journal of Zoology

Research Article

Genetic Characterization of Six Stocks of Litopenaeus vannamei Used in Cuba for Aquaculture by Means of Microsatellite Loci

Table 1

Genetic variability parameters and the Hardy-Weinberg deviation of equilibrium () and its probability (Pfis) of four microsatellites loci isolated from L. vannamei, M-1 [10], Pvan 0040, Pvan 1758 and Pvan 1815 [11], in six different cultured stocks of L. vannamei used for aquaculture in Cuba. n: number of samples. Na: number of alleles, Ne: number of effective alleles, Np: number of private alleles, He: expected heterozygosity, Ho: observed heterozygosity. The associated probability was estimated using the Markov chains (10000 Dememorisation, 100 batches, and 5000 iterations per batch using Genepop program Version 4.1.0. Numbers in parenthesis are standard mean error.


Stock	N	Na	Ne	Np	Ho	He	Fis	Pfis

Locus M1

S2-1	19	7	4.6	0	0.89	0.78	−0.115	0.001
S1-2	19	4	2.2	0	0.62	0.55	−2.212	0.660
S1-3	16	5	3.6	0	0.69	0.72	0.078	0.000
S1-4	22	3	2.0	0	0.59	0.50	−0.147	0.142
S1-1 × S1-3	23	5	3.0	0	0.91	0.67	−0.349	0.242
S1-2 × S1-3	25	4	3.1	0	0.36	0.68	0.485	0.000

Locus Pvan 0040

S2-1	21	4	2.4	0	0.52	0.58	0.127	0.002
S1-2	21	2	1.3	0	0.24	0.21	−0.111	1.000
S1-3	19	4	2.5	0	0.16	0.59	0.746	0.000
S1-4	22	3	2.7	0	0.36	0.63	0.440	0.000
S1-1 × S1-3	23	4	3.9	0	0.56	0.74	0.261	0.000
S1-2 × S1-3	26	2	1.3	0	0.23	0.20	-0.111	1.000

Locus 1758

S2-1	21	7	3.8	0	0.67	0.54	0.121	0.00
S1-2	21	4	3.5	0	0.67	0.71	0.088	0.000
S1-3	20	9	6.2	3	0.75	0.84	0.132	0.054
S1-4	22	7	3.9	1	0.68	0.74	0.108	0.009
S1-1 × S1-3	23	6	4.5	0	0.61	0.78	0.239	0.119
S1-2 × S1-3	26	6	1.8	0	0.73	0.64	-0.116	0.563

Locus 1815

S2-1	21	3	2.1	0	0.14	0.52	0.737	0.00
S1-2	21	4	1.6	0	0.25	0.37	0.371	0.056
S1-3	20	6	3.8	0	0.65	0.74	0.147	0.006
S1-4	22	6	4.4	1	0.73	0.77	0.084	0.000
S1-1 × S1-3	23	8	3.8	0	0.52	0.74	0.315	0.000
S1-2 × S1-3	26	4	3.6	0	0.65	0.72	0.115	0.000

All loci

S2-1	20.5(0.5)	5.2 (1.0)	3.2 (0.6)	0	0.56 (0.16)	0.66 (0.06)	0.176	0.000
S1-2	20.5 (0.5)	3.5 (0.5)	2.1 (0.5)	0	0.46 (0.13)	0.46 (0.11)	0.032	0.000
S1-3	18.7 (0.9)	6.0 (1.0)	4.0 (0.8)	3	0.56 (0.14)	0.72 (0.05)	0.250	0.000
S1-4	22.0 (0.0)	4.7 (1.0)	3.3 (0.5)	2	0.59 (0.08)	0.66 (0.06)	0.132	0.000
S1-1 × S1-3	23.0 (0.0)	5.0 (0.8)	3.8 (0.3)	0	0.65 (0.90)	0.73 (0.02)	0.131	0.000
S1-2 × S1-3	25.7 (0.2)	4.0 (0.8)	2.7 (0.5)	0	0.50 (0.12)	0.56 (0.12)	0.141	0.000