Noninvasive Method for a Statewide Survey of Eastern Hellbenders Cryptobranchus alleganiensis Using Environmental DNA

Table 1

Detection of Hellbender cyt b gene eDNA in static zoo water and stream water harboring a documented Hellbender in a Southeastern Ohio watershed.

Location	Site number	Liters	Hellbender cyt b DNA +/−
Columbus Zoo Hellbender Tank	A1	1	+
	A2	2	+
	B1	1	+
	B2	2	+
	C1	1	−
	C2	2	+
Southeastern Ohio Watershed	A1	1	−
	B2	1	−
	C2	1	−
	A3	1	−
	A1	2	+
	A2	2	+
	B1	2	+
	B2	2	+
	C1	2	+
	C2	2	+
Environmental negative control		2	−