Blood Cell Profile of the Developing Tadpoles and Adults of the Ornate Frog, Microhyla ornata (Anura: Microhylidae)

Table 1

Morphometry of erythrocytes during the larval development and in the adults of the ornate frog, Microhyla ornata.

Stages		STL	Size of cell				Size of nuclei				A ′/A ± SD
Gosner	Taylor and Kollros		Length (L ± SD) (μm)	Breadth (B ± SD) (μm)	L/B ± SD	Area (A ± SD) (μm²)	Length; (𝐿^′±SD) (μm)	Breadth; (𝐵^′±SD) (μm)	L ′/B′ ± SD	Area (𝐴^′±SD) (μm²)
26	I	1.5±0.0	16.05±2.22	17.27±2.89	0.92±0.11	218.45±48.11	9.09±1.65	9.39±1.32	0.96±0.06	68.10±20.06	0.31±0.03
27	II	1.5±0.0	19.24±1.73	17.72±1.98	1.08±0.11	267.46±36.32	10.60±1.79	9.39±1.63	1.12±0.06	78.58±21.62	0.29±0.03
28	III	1.7±0.0	17.11±2.46	16.51±1.78	1.03±0.11	221.67±35.81	9.39±1.32	9.69±1.81	0.96±0.06	71.36±16.58	0.32±0.03
29	IV	1.7±0.04	16.66±2.24	16.81±1.50	0.99±0.11	219.50±31.70	9.39±1.32	9.24±0.66	1.01±0.06	68.06±10.32	0.31±0.03
30	V	1.7±0.04	19.08±2.55	18.93±2.11	1.00±0.11	282.00±41.94	10.30±2.00	10.30±1.4	1.00±0.06	83.97±23.28	0.29±0.03
31	VI	1.7±0.12	18.78±1.54	18.18±1.35	1.03±0.11	267.80±26.14	9.24±1.16	8.63±1.73	1.07±0.06	62.35±13.53	0.23±0.03
32	VII	1.7±0.09	15.45±3.69	15.60±3.07	0.99±0.11	182.78±29.76	7.72±1.50	8.18±1.68	0.94±0.06	48.66±10.40	0.26±0.03
33	VIII	1.8±0.09	17.57±1.81	16.36±1.76	1.07±0.11	224.58±23.16	9.84±1.31	9.24±0.66	1.06±0.06	71.36±9.92	0.31±0.03
34	IX	1.9±0.04	16.65±1.51	17.57±1.81	0.97±0.11	229.96±31.73	9.39±0.90	9.39±0.90	1.00±0.06	69.90±15.13	0.30±0.03
35	X	1.8±0.0	15.75±1.54	15.60±1.98	1.00±0.11	192.48±26.42	7.72±1.50	8.02±1.44	0.96±0.06	48.65±13.19	0.25±0.03
36	XI	2.0±0.0	15.75±1.54	15.30±2.24	1.02±0.11	193.19±33.63	9.24±0.66	8.33±1.31	1.10±0.06	60.54±11.02	0.31±0.03
37	XII	2.5±0.0	16.65±1.51	15.90±1.31	1.04±0.11	208.68±30.02	9.09±1.35	8.02±1.44	1.13±0.06	57.66±14.94	0.27±0.03
38	XIII	2.4±0.16	16.36±1.76	16.21±1.73	1.00±0.11	207.61±27.97	8.93±1.50	8.18±1.38	1.09±0.06	57.66±14.94	0.27±0.03
39	XIV	2.4±0.18	16.66±2.24	16.66±1.51	1.00±0.11	216.30±22.43	8.78±1.63	8.18±1.38	1.07±0.06	56.94±15.93	0.26±0.03
40	XV-XVII	2.2±0.2	18.02±1.78	16.66±1.79	1.08±0.11	235.74±33.12	8.33±1.31	7.72±1.78	1.07±0.06	51.53±16.94	0.21±0.03
41	XVIII-; XIX	2.3±0.04	17.87±1.63	16.51±2.02	1.08±0.11	231.03±30.33	8.18±1.38	7.42±1.50	1.10±0.06	47.93±13.53	0.27±0.03
42	XX	2.2±0.0	15.30±2.94	17.26±3.96	0.88±0.11	195.40±42.44	7.42±1.50	7.72±1.50	0.96±0.06	45.05±12.86	0.22±0.03
43	XXI	1.4±0.04	15.90±3.81	15.30±3.64	1.03±0.11	182.06±22.90	8.63±1.11	7.42±1.50	1.16±0.06	50.81±13.56	0.290±0.03
44	XXII	1.2±0.0	19.99±2.60	13.78±3.10	1.45±0.11	211.27±24.24	8.93±0.66	8.18±1.38	1.09±0.06	57.66±11.38	0.27±0.03
45	XXIII-; XXIV	0.7±0.08	15.45±2.69	18.48±2.69	0.83±0.11	219.56±21.44	8.48±1.21	8.78±0.90	0.96±0.06	58.38±9.89	0.26±0.03
46	XXV	0.6±0.0	16.05±1.38	16.21±1.73	0.99±0.11	204.02±24.36	8.18±1.38	7.59±1.51	1.07±0.06	48.65±12.86	0.23±0.03
Correlation coefficient (𝑟)			−0.208	−0.348	+0.124	−0.438	−0.591	−0.758	+0.296	−0.713	−0.591
Sex (no. of adult frogs used)	Average body weight (g) ± SD	Average SVL (cm) ± SD	Size of cell				Size of nuclei				𝐴^′/𝐴 ± SD
			Length (L ± SD ) (μm)	Breadth (B ± SD) (μm)	L/B ± SD	Area (𝐴 ± SD) (μm²)	Length; (𝐿^′±SD) (μm)	Breadth; (𝐵^′±SD) (μm)	𝐿^′/𝐵^′ ± SD	Area (𝐴^′±SD) (μm²)
Males (7)	10.12 ± 0.84	1.8 ± 0.31	22.11 ± 2.16	13.18 ± 2.20	1.73 ± 0.34	254.39 ± 40.82	12.27 ± 1.16	6.81 ± 1.62	1.86 ± 0.32	65.96 ± 18.13	0.265 ± 0.08
Females (7)	11.86 ± 0.73	1.9 ± 0.12	21.96 ± 2.11	13.93 ± 2.22	1.62 ± 0.32	238.29 ± 29.75	12.42 ± 1.63	7.72 ± 1.78	1.66 ± 0.31	76.43 ± 23.51	0.319 ± 0.08

STL: snout to tail tip length; SVL: snout to vent length; SD: standard deviation; 𝜇m: micrometer.