Studies on the Pinctada fucata BMP-2 Gene: Structural Similarity and Functional Conservation of Its Osteogenic Potential within the Animal Kingdom

Figure 3

Effect of the recombinant mature domain of PfBMP-2 and the synthetic polypeptide of the knuckle epitope of human BMP-2 treatment on the endogenous expression of alkaline phosphatase in C3H10T1/2 cells. C3H10T1/2 cells were plated into 48-well tissue culture dishes for 2 days followed by incubation with the recombinant mature BMP-2 and the synthetic polypeptide of the Knuckle epitope of human BMP-2 at a final concentration of 5 μg/well and 80 μg/well, respectively. At 7, 11, and 21 days, alkaline phosphatase expression was measured, as indicated. ⬤-⬤: PfBMP-2; ■-■: the Knuckle epitope of human BMP-2 synthetic polypeptide; ◆-◆: in the absence of protein.