Spermatogenic Waves and Expression of AR and ERs in Germ Cells of Podarcis sicula

Figure 3

Histology and mRNA distribution of AR and ERs in the testis during the autumnal recrudescence. (a) Mallory’s trichrome staining: all stages of germ cells differentiation from spg to spz are evident in the seminiferous epithelium as in the mating period. (b, c, d) ISH with homologous probes: intense positivity to AR (b), ERα (c), or ERβ (d) probes of the spg, on the basis of the tubules, and of spcII near the lumen (); the spcI in pre-leptotene constitute a large negative circular ring where only few pachitene cells (arrows) are slightly positive. The bar is 30 µm.

(a)

(b)

(c)

(d)