Descriptive Osteology of a Newly Described Stone Loach, Oxynoemacheilus chomanicus (Kamangar et al., 2014) (Cypriniformes, Nemacheilidae)
Oxynoemacheilus chomanicus is a newly described species of the family Nemacheilidae from the Choman River drainage of the Tigris basin. This study was conducted to provide the detailed osteological characteristics of this species and comparing them with those of other endemic species of the genus Oxynoemacheilus from inland water basins of Iran, namely, kiabii, O. persa, O. brandtii, O. kermanshahensis, and O. bergianus. For this purpose, nine specimens of O. chomanicus were collected, cleaned, and stained for osteological examination. Then, a detailed description of their skeletal structure was provided. The results showed that O. chomanicus can be distinguished from other studied species of the genus Oxynoemacheilus due to possessing an orbital shelf, number of the vertebrae, number of the hypurals, number of the unbranched rays in anal fin, features of the hemal and neural spines, and connection pattern of the parietal and frontal.
Stone loaches, the family Nemacheilidae, are small benthic fishes  found in fresh waters of Asia and its islands, Europe, and northeast Africa . This family have a great diversity in Iranian inland waters [1, 3, 4], with more than 40 reported species, including 24 endemic species [3–9]. They are less known due to small size and low economic value [10, 11] and their classification is still complicated; therefore, ichthyologists are trying to reveal their phylogenetic status . Given that, the osteological characteristics can play an important role in this regard , since osteology is a useful tool to study the taxonomy and phylogenetic relationships among fishes [14, 15].
The genus Oxynoemacheilus is a species rich genus of the family Nemacheilidae known from Albania eastwards to central Iran  and has 11 reported species from Iran of which three include O. chomanicus, O. kurdestanicus, and O. zagrodensesis recently described from the Choman River drainage of the Tigris basin . The newly described species of O. chomanicus is found in the Choman River drainage (Baneh, Kurdistan Province). Identification of this species is based on the morphological features that show many similarities to other members of this genus .
Regarding high diversity and morphological similarity of the members of the family Nemacheilidae, using combined data, including osteological and molecular data, can help to better understand their taxonomical relationship. Therefore, this study was conducted to provide detailed osteological characteristics of O. chomanicus and comparing them with those of other species of the genus Oxynoemacheilus from the inland water basins of Iran that their osteological data are available. The finding of this study can provide the osteological features of this species that can help to discrete this species from others and be used as a basis for further phylogenetic study of the members of this genus based on the osteological data.
2. Material and Methods
Nine specimens of O. chomanicus with standard length of (mean ± SD) were collected using electrofishing device from the Choman River (Kurdistan Province, Iran). Then, specimens were anesthetized with 1% clove solution and fixed in 4% formaldehyde. For osteological examination, the specimens were cleared and stained using alcian blue and alizarin red based on Taylor and Van Dyke . Pictures of the stained skeletal structures were obtained using an Epson V600 scanner equipped with a glycerol bath. The skeletal structures of each sample were observed and studied by an MS5 Leica stereomicroscope. The scanned images were illustrated by CorelDraw X6 software. Nomenclature and abbreviation of the skeletal elements follow Prokofiev [12, 18]. Detailed descriptions of the osteological features of O. kiabii, O. bergianus, O. persa, O. brandtii, and O. kermanshahensis were provided by Mafakheri et al. , Jalili and Eagderi , Mafakheri et al. , and Mafakheri , respectively.
The anterior part of the neurocranium is narrow with wider posterior part and its maximum width is about 60% of its length. The ethmoid region consists of the paired lateral ethmoids and unpaired supraethmoid-ethmoid and prevomer. The elongated supraethmoid-ethmoid bone is vertically fused to the prevomer and posteriorly sutured with the frontal (Figures 1(a) and 1(b)). The anterior part of the prevomer is heart-shaped (Figure 1(b)) and is posteriorly connected to the orbitosphenoid (Figure 1(c)). The lateral ethmoid is medially attached to the orbitosphenoid (Figure 1). The anterior part of the lateral ethmoid is projected and its posterior part is convex in shape (Figure 1).
The orbital region includes the paired frontal, pterosphenoid, and sclerotic, and the unpaired orbitosphenoid and parasphenoid. The frontal is the largest bony element of the neurocranium’s roof. This bone is laterally connected to the orbitosphenoid, pterosphenoid, and sphenotic and posteriorly to the parietal (Figures 1(a) and 1(b)). The frontal is connected to the lateral ethmoid via the orbital shelf (Figure 1(a)). The two frontals are connected medially but they are separated posteriorly and form the anterior part of the fontanelle (Figure 1(a)). The orbitosphenoid is posteriorly connected to the heavy bone of the pterosphenoid. In the ventral plan, the parasphenoid is connected to the prevomer, orbitosphenoid, pterosphenoid, prootic, and basioccipital (Figure 1(c)). The parasphenoid, orbitosphenoid, and pterosphenoid enclose the orbital foramen (Figure 1(b)). The parasphenoid meets the prootic and pterosphenoid via its lateral wings and form a large foramen in ventral plan of the neurocranium (Figure 1(c)). Two small bones of the sclerotic enclose the orbits.
The otic region consists of the parietals, epiotics, pterotics, prootics, and sphenotics (Figure 1). The parietal is located between the frontal and supraoccipital (Figure 1(a)). This bone is anterolaterally connected to the sphenotic and posterolaterally to the epiotic (Figures 1(a) and 1(b)). The pterotic is almost triangle in shape that it locates between the sphenotic and epiotic dorsally, and the prootic and exoccipital ventrally (Figures 1(a) and 1(c)). Connection of the epiotic and sphenotic avoids the relationship between the pterotic and parietal (Figure 1(b)). The pterosphenoid, sphenotic, and prootic form the anterior articulatory facet for articulating the anterior condyle of the hyomandibular and the sphenotic and pterotic form the posterior articulatory facet for articulating the posterior condyle of the hyomandibular (Figures 2(b) and 2(c)).
The occipital region consists of both unpaired supraoccipital and basioccipital and the paired exoccipital (Figure 1). The supraoccipital forms the posterior part of the fontanelle. This bone is connected to the sphenotic by its anterolateral wings and posteriorly to the exoccipitals (Figure 1(a)). The exoccipital bears a large foramen and it is connected to the prootic anteroventrally (Figure 1(c)). The basioccipital is posteriorly narrower having a ring-shaped process (Figure 1(c)). This bone is situated between the exoccipitals and connected to the prootics anteriorly (Figure 1(c)). The basioccipital is posteriorly articulated to the first centrum. In a ligamentous space anterior to the ethmoid region, the paired preethmoid II, prepalatine, and sesamoid and unpaired kinethmoid are present (Figure 2). The prepalatine is located between the autopalatine and maxilla. The prepalatine is medially connected to the preethmoid II (Figure 2(a)). The rod-shaped preethmoid II is anteriorly connected to the maxilla and posteriorly to the prevomer. The cylindrically shaped kinethmoid is vertically located between jaws (Figure 2(a)).
The upper jaw consists of the premaxilla and maxilla (Figure 3). The premaxilla has two processes, that is, pr. ascenden and pr. alveolar. The premaxilla is ligamentously connected to the kinethmoid. The maxilla is a large laminar bone which has a small anteroventral process connecting to the preethmoid II and prepalatine and an anteroventral process that is tilted downwardly reaching its counterpart (Figure 2(a)). The lower jaw is composed of the dental, articular, retroarticular, and coronomeckelian. The large dental has two processes, including narrow ramus dentalis and wide coronoid processes. The articular is overlapped with the dental and posteriorly articulated to the quadrate. The small bone of the coronomeckelian is attached to the medial face of the articular. The small bone of the retroarticular is articulated to the posteroventral part of the articular.
3.3. Branchial Apparatus
The branchial arch consists of the basibranchials, hypobranchials, ceratobranchials, epibranchials, and infrapharyngobranchials (Figure 4). There are four basibranchials, which locate between the hypobranchials. The two first basibranchials are similar in shape. The hypobranchials are three pairs and almost square-shaped. In each side, there are five ceratobranchials of which the last one has different shape possessing pharyngeal teeth (Figure 4). The four paired epibranchials situate between the rectangularly shaped ceratobranchials and the small infrapharyngobranchials. The infrapharingobranchials are horizontally arranged on the epibranchials.
3.4. Hyoid Arch
The hyoid arch is composed of the basihyal, dorsal hypohyal, ventral hypohyal, ceratohyal, epihyal, interhyal, urohyal, extra urohyal, and branchiostegals (Figure 5). The large cylindrical ceratohyal intervenes between the hypohyals and the epihyal (Figure 5). The epihyal is triangular in shape. A small rod-shaped interhyal fits in a small groove on the posterodorsal part of the epihyal ventrally. The dorsal hypohyal and ventral hypohyal are connected firmly. Two small rod-shaped extra urohyals are located between the hypohyals and ligamentously connected to the urohyal and basihyal. The laminar bone of the urohyal bears a depression anteriorly and wings anterolaterally (Figure 5). The unpaired basihyal is T-shaped. There are three curved branchiostegals of which the first one is attached to the ceratohyal, the connection point of the second one is located between ceratohyal and epihyal, and the third one is connected to the epihyal.
The suspensorium consists of the autopalatine, endopterygoid, ectopterygoid, metapterygoid, hyomandibular, quadrate, and symplectic (Figure 6). The autopalatine is a heavy bone articulating to the endopterygoid posteriorly (Figure 2(b)). There is a strong ligamentous attachment between the endopterygoid, ectopterygoid, metapterygoid, and quadrate. The triangle-shaped bone of the symplectic is inserted into the depression of the quadrate. A condyle for articulation to the articular presents in the anteroventral part of the quadrat. The hyomandibular bears two articular heads dorsally for the craniohyomandibular articulations.
3.6. Opercular Series
This series consists of the opercle, subopercle, interopercle, and preopercle (Figure 6). The opercle is the largest element of the opercular series and its dorsal margin is concaved. This bone has a large condyle, which is articulated to the hyomandibular. The opercle covers the paddle-shaped subopercle ventrally (Figure 7). The narrow curved preopercle almost locates at the rear of the interopercle (Figure 6). The interopercle is not completely ossified and connected to the subopercle posteriorly (Figure 6).
3.7. Pectoral Girdle
The pectoral girdle includes the cleithrum, coracoid, scapula, mesocoracoid, supratemporal, posttemporal, supracleithrum, and radials (Figure 7). The cleithrum is the largest bony elements of the pectoral girdle possessing the vertical and horizontal sections. Its vertical section is connected to the supracleithrum, whereas the horizontal one is connected to the scapula and coracoid. The coracoid is narrow anteriorly. The scapula is located between the cleithrum and coracoid. There is a large foramen on its middle face. The mesocoracoid is a small rod-shaped bone, which is medially located between the cleithrum and coracoid. The mesocoracoid indirectly connects these two bones (Figure 7). The supracleithrum bears a ridge on its lateral face. The elongated posttemporal is situated in the anterolateral depression of the supracleithrum. This bone is posterolaterally fused to the supratemporal. The supratemporal is a small tubular-shaped bone. There are four radials of which first two ones are narrow and elongated and the most medial one is the smallest and widest one.
3.8. Pelvic Girdle
The pelvic girdle consists of the paired pelvic bones and radials. The pelvic bones are horizontally located in belly area. The pelvic bones are firmly connected by a strong ligament medially. They have posterolateral processes, that is, pr. iliacus that along with the posterior process, that is, pr. Ischiadicus, forms a depression for positioning the rays and radials. The pelvic bone is anteriorly narrow (pubic process). There are three small radials. There are one unbranched ray, seven branched rays, and one small curved pelvic splint in the pelvic fin.
3.9. Axial Skeleton and Unpaired Fins
There are 37 centra of which first four centra form the Weberian apparatus and bony capsule. The hemal and neural spines are narrow. The dorsal fin has 9 pterygiophores and one stay and the first pterygiophore is the largest reaching the 11th or 12th vertebra. There are five unbranched and 81/2 branched rays in dorsal fin (Figure 8(a)). The number of the pterygiophores in anal fin is six with one stay in which first pterygiophore reaches the 24th centrum. The number of unbranched and branched rays in anal fin is 4 and 51/2, respectively (Figure 8(b)).
3.10. Caudal Skeleton
The caudal complex consists of the second preural centrum and its neural and hemal processes, first preural centrum, ural-1+ural-2, pleurostyle, hypurals, parhypural, epural, and principal caudal rays, and procurrent rays (Figure 9). The hemal process of the second preural is articulated to its centrum and its neural process is fused to its centrum. The last centrum is formed by the fused ural-1, ural-2, and preural-1. The wide rectangularly shaped parhypural along with the hypural-1 is articulated to the last centrum. The parhypural is dorsally connected to the hypural-1. There are six hypurals of which first two ones are large. The hypural-2 is fused to the centrum and others are inserted into the groove of the pleurostyle. The last hypural is small. The small epural is located between the pleurostyle and neural rudimentary. The pleurostyle is elongated and fused to the centrum. The principal caudal rays are supported by the hypurals.
The members of the genus Oxynoemacheilus, with 11 reported species, are distributed in the most of the inland waters basins of Iran and their identification is difficult based on their external morphology. Hence, the osteological features are suitable characteristics for their distinction [12, 18]. In addition, the osteological data can be well interpreted in taxonomy and phylogenetic relationships of fish species even in higher level . There are various works on the phylogenetic relationships of the superfamily Cobitoidea using osteological characteristics containing important information [12, 13, 18, 24–27]. Therefore, the present study provided detailed osteological characteristics of O. chomanicus for further taxonomical studies of these taxa.
Prokofiev  has pointed out that the coronomeckelian is located on the base and dorsal edge of the coronoid processes in the genus Oxynoemacheilus, whereas it is attached to the articular in O. chomanicus as mentioned by Sawada . In addition, Prokofiev  mentioned that the lateral ethmoid is stationarily jointed with the supraethmoid-ethmoid, while such a feature was not observed in O. chomanicus. In O. chomanicus, similar to other compared species of this genus, the prootic, pterosphenoid, and sphenotic form the anterior articulatory facet, and the pterotic and sphenotic form the posterior articulatory facet, whereas Prokofiev  mentioned that, in the members of the genus Oxynoemacheilus, the anterior articulatory facet is restricted by the sphenotic and prootic, and the posterior articulatory facet is restricted by the sphenotic, prootic, and pterotic.
Based on the results, this species shows some osteological features that can be considered as its osteological identification key in comparison to O. kiabii , O. persa , O. brandtii, O. kermanshahensis , and O. bergianus . There is an orbital shelf of the frontal in O. chomanicus, whereas such shelf is not present in O. persa . The posterior process of the orbitosphenoid in O. persa is well developed  unlike that of O. chomanicus.
In O. kiabii, the supraethmoid-ethmoid is shorter and wider  than that of O. chomanicus. The connection pattern of the frontal and parietal in O. kiabii had a zigzag pattern  versus straight one of O. chomanicus. Furthermore, there is a small bone between the frontal and parietal in O. persa . The shape of the metapterygoid in O. chomanicus is rectangular, while it is square-shaped in O. kiabii . Furthermore, the hypohyals’ processes of O. kiabii are well developed  unlike that of O. chomanicus. In O. brandtii and O. bergianus, the hemal and neural spines are wider and well developed [20, 22], while those of O. chomanicus are thin and narrow similar to O. kiabii, O. persa, and O. bergianus.
Oxynoemacheilus chomanicus has six hypurals, whereas O. kiabii, O. brandtii, O. persa, and O. kermanshahensis have five hypurals. The number of the vertebrae in O. chomanicus is 37, whereas O. persa and O. kermanshahensis have 34-35 and 39-40 vertebrae, respectively [21, 22]. The dorsal margin of the maxilla in O. chomanicus is smooth, whereas it is convex-shaped in O. kermanshahensis .
In O. chomanicus, the number of unbranched rays in dorsal and anal fins is five and four, respectively, which is different from the original description of this species (three and three) by Kamangar et al. . These extra unbranched rays were visible only in cleared and stained specimens. The number of branched rays in dorsal and anal fins of O. chomanicus is 81/2 and 51/2, respectively, corresponding to description of this species by Kamangar et al. . The branched rays of the dorsal fin in O. chomanicus are more than those of O. kermanshahensis .
Some species of the genus Oxynoemacheilus were previously placed in other genera, including Barbatula and Triplophysa, but osteological investigations revealed their differences and ascribed them to related genera [12, 13]. Of the most important differences of the genus Oxynoemacheilus with the members of the genera Barbatula and Triplophysa are lack of the preethmoid-I and the number and shape of the radials of the pectoral fin. In the examined species, including O. persa, O. bergianus, O. brandtii, O. kermanshahensis, O. kiabii, and O. chomanicus, the preethmoid-I is absent. The numbers of the radials in the pectoral fins of Triplophysa is 3 and Barbatula is 4 with different shape compared to the members of the genus Oxynoemacheilus. The members of the genus Oxynoemacheilus bear two small sesamoid bones, whereas this bone is absent in Triplophysa and Barbatula [12, 13]. In Oxynoemacheilus, the number of the branched rays of the anal fin is 5, whereas this number can be higher in Barbatula and Triplophysa.
Based on the results, the presence of the orbital shelf, number of the vertebrae, number of the hypurals, number of the unbranched rays in anal fin, features of the hemal and neural spines, and connection pattern of the parietal and frontal can be offered as identified keys for O. chomanicus from other examined species of the genus Oxynoemacheilus from inland water of Iran.
Conflict of Interests
The authors declare that there is no conflict of interests regarding the publication of this paper.
K. Golzarianpour, A. Abdoli, and J. Freyhof, “Oxynoemacheilus kiabii, a new loach from Karkheh River drainage, Iran (Teleostei: Nemacheilidae),” Ichthyological Exploration of Freshwaters, vol. 22, no. 3, pp. 201–208, 2011.View at: Google Scholar
B. Coad, Fresh Water Fishes of Iran, 2014, http://www.briancoad.com.
A. Abdoli, K. Golzarianpour, B. Kiabi, M. Naderi, and R. Patimar, “Status of the endemic loaches of Iran,” Folia Zoologica, vol. 60, no. 4, pp. 362–367, 2011.View at: Google Scholar
H. R. Esmaeili, B. W. Coad, A. Gholamifard, N. Nazari, and A. Teimory, “Annotated checklist of the freshwater fishes of Iran,” Zoosystematica Rossica, vol. 19, pp. 361–386, 2010.View at: Google Scholar
H. R. Esmaeili, G. Sayyadzadeh, M. Özulug, M. Geiger, and J. Freyhof, “Three new species of Turcinoemacheilus from Iran and Turkey (teleostei: Nemacheilidae),” Ichthyological Exploration of Freshwaters, vol. 24, no. 3, pp. 257–273, 2014.View at: Google Scholar
J. Freyhof, H. R. Esmaeili, G. Sayyadzadeh, and M. Geiger, “Review of the crested loaches of the genus Paracobitis from Iran and Iraq with the description of four new species (Teleostei: Nemacheilidae),” Ichthyological Exploration of Freshwaters, vol. 25, no. 1, pp. 11–38, 2014.View at: Google Scholar
J. Freyhof, G. Sayyadzadeh, H. R. Esmaeili, and M. Geiger, “Review of the genus Paraschistura from Iran with description of six new species (Teleostei: Nemacheilidae),” Ichthyological Exploration of Freshwaters, vol. 26, no. 1, pp. 1–48, 2015.View at: Google Scholar
H. Mousavi-Sabet, G. Sayyadzadeh, H. R. Esmaeili, S. Eagderi, R. Patimar, and J. Freyhof, “Paracobitis hircanica, a new crested loach from the southern Caspian Sea basin (Teleostei: Nemacheilidae),” Ichthyological Exploration of Freshwaters, vol. 25, no. 4, pp. 339–346, 2015.View at: Google Scholar
A. Abdoli, The Inland Water Fishes of Iran, Iranian Museum of Nature and Wildlife, Tehran, Iran, 2000.
M. Kottelat, Indochinese Nemacheilines: A Revision of Nemacheiline Loaches (Pisces: Cypriniformes) of Thailand, Burma, Laos, Cambodia, and Southern Viet Nam, Verlag Dr. Friedrich Pfeil, München, Germany, 1990.
Y. Sawada, “Phylogeny and zoogeography of the superfamily Cobitoidea (Cyprinoidei, Cypriniformes),” Memoirs of the Faculty of Fisheries of Hokkaido University, vol. 28, pp. 65–223, 1982.View at: Google Scholar
P. Jalili, S. Eagderi, N. Nikmehr, and Y. Keivany, “Descriptive osteology of Barbus cyri (Teleostei: Cyprinidae) from southern Caspian Sea basin Iran,” Iranian Journal of Ichthyology, vol. 2, no. 2, pp. 105–112, 2015.View at: Google Scholar
J. Freyhof, F. Erk'akan, C. Özeren, and A. Perdices, “An overview of the western Palaearctic loach genus Oxynoemacheilus (Teloestei: Nemacheilidae),” Ichthyological Exploration of Freshwaters, vol. 22, no. 4, pp. 301–312, 2011.View at: Google Scholar
W. R. Taylor and G. C. Van Dyke, “Revised procedures for staining and clearing small fishes and other vertebrates for bone and cartilage study,” Cybium, vol. 9, pp. 107–119, 1985.View at: Google Scholar
P. Mafakheri, S. Eagderi, H. Farahmand, and H. Mousavi-Sabet, “Osteological structure of kiabi loach (Oxynoemacheilus kiabii),” Iranian Journal Ichthyology, vol. 3, pp. 197–205, 2014.View at: Google Scholar
P. Jalili and S. Eagderi, “Osteology of Safidrud stone loach Oxynoemacheilus bergianus,” Journal of Animal Researches, vol. 28, pp. 21–34, 2015.View at: Google Scholar
P. Mafakheri, S. Eagderi, H. Farahmand, and H. Mousavi-Sabet, “Descriptive osteology of Persian loach (Oxynemacheilius persa),” Taxonomy and Biosystematics, vol. 73, no. 3, pp. 115–123, 2015.View at: Google Scholar
P. Mafakheri, Phylogenetic of Iranian genus Oxynoemacheilus (Nemacheilidae) using morphological characters [M.S. thesis], University of Tehran, 2014.
L. A. Rojo, Dictionary of Evolutionary Fish Osteology, CRC Press, 1991.
S. L. Hora, “Note on homalopterid fishes in the collections of certain American museums,” Records of the Indian Museum, vol. 48, pp. 45–57, 1950.View at: Google Scholar
S. L. Hora, “Classification, bionomics and evolution of homalopterid fishes,” Memoirs of Indian Museum, vol. 12, pp. 263–330, 1932.View at: Google Scholar
L. S. Ramaswami, “Skeleton of cyprinoid fishes in relation to phylogenetic studies. V. The skull and the gasbladder capsule of the Cobitidae,” Proceedings of the National Institute of Sciences of India, vol. 18, pp. 519–538, 1953.View at: Google Scholar