In Vitro Antibacterial Potential of Extracts of Sterculia africana, Acacia sieberiana, and Cassia abbreviata ssp. abbreviata Used by Yellow Baboons (Papio cynocephalus) for Possible Self-Medication in Mikumi National Park, Tanzania
In the animals in general and nonhuman primates in particular self-medication has been widely reported; however, little is still known about the pharmacological activity of the extracts present in their daily diet. The in vitro antibacterial activity of the stem, root bark, and leaf extracts of three selected plants on which yellow baboons feed in an unusual manner in Mikumi National Park, Tanzania, was evaluated. Crude plant extracts were tested against Gram positive and Gram negative bacteria of medical and veterinary importance employing a modified agar well diffusion method and Minimum Inhibitory Concentration (MIC) technique. The lowest MIC value for Gram positive strains was 0.31 mg/ml demonstrated by Cassia abbreviata ssp. abbreviata against Staphylococcus aureus (ATCC 25923). The highest susceptibility to the ethanol plant extracts was exhibited by Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus, examples of microbes that affect both human and nonhuman primates. These findings demonstrate that the plant extracts from Sterculia africana, Acacia sieberiana, and Cassia abbreviata ssp. abbreviata have antibacterial activity and may be used as feed for their prophylactic benefits. Remarkably, the lowest MIC of 0.16 mg/ml was only 16-fold weaker than Gentamicin, a standard drug.
There is a growing body of evidence to support the theory that animals are self-medicated by ingesting plants of both nutritional and medicinal value [1–5]. A Tanzanian medicine man, Babu Kalunde, almost a century ago, was able to treat a dysentery-like illness by observing a porcupine with similar symptoms ingesting the roots of mulengelele, a plant previously believed to be toxic . Some of the evidence for self-medication by African great apes includes the infrequent intake of plant species which are not a regular part of the diet, restriction of plant use to seasons or other periods associated with high risk of parasitic infection [5–7], illness, or parasite infection of the individual at the time of ingestion of a putative medicinal plant [5, 6, 8], and a subsequent positive change in this condition following ingestion [7, 9, 10]. Furthermore, it has been suggested that compounds found in the ordinary diet of animals may have important positive effects on health and may prevent risks of infection and illness [2, 11]. Chemical investigations of the self-medication hypothesis have found that the bitter pith of Vernonia amygdalina has chemical compounds that apparently are responsible for the control of nematode infections . Diospyros abyssinica, Uvariopsis congensis, Albizia grandibracteata, and Trichilia rubescens are only a few of the plants present in the nonprimate diet that have been tested for biological activity [13, 14].
Among the plants eaten by yellow baboons (Papio cynocephalus) inside Mikumi National Park, Tanzania, are Acacia sieberiana (Mimosaceae family), Sterculia africana (Sterculiaceae family), and Cassia abbreviate ssp. abbreviata (Caesalpiniaceae family), the focus of this study. The leaves and stem bark of Acacia sieberiana and the stem bark of Cassia abbreviata ssp. abbreviata and Sterculia africana were observed to be feed of the yellow baboons. On three separate occasions while observing yellow baboons, only one or two, as opposed to the whole group, would stop and feed on the leaves or stem bark of these plants and then join up with the rest of the group. A video of this phenomenon was recorded on one of these visits. However, though not observed to be consumed, the roots of all three plants and leaves of Cassia abbreviata ssp. abbreviata were analysed as well.
A number of studies have been conducted on Cassia abbreviata Oliv. , a close relative of Cassia abbreviata ssp. abbreviata, endemic to Morogoro region from where this sample was taken [16, 17]; however there is a dearth of literature on Cassia abbreviata ssp. abbreviata. In Bukoba and Morogoro, Tanzania, the root bark of Cassia abbreviata ssp. abbreviata is used in the treatment of oral and vaginal candidiasis particularly in HIV/AIDS patients [18, 19].
Sterculia africana is valued in the study area for traditional worship and is one of the plants associated with ancestral sacrifices in Tanzania . Sterculia africana root is used to treat asthma in communities around Lake Victoria region in Tanzania  and also possesses strong antifungal activity . The Maale and Ari communities in Ethiopia utilize Sterculia africana as an antiemetic and to treat food poisoning as well as treating fever in the Blue Nile state, Sudan [22, 23]. Sterculia africana is one of the sources of nontraditional seed oils in Botswana  and is widely used in African traditional medicine. In Somalia a decoction of the crushed fresh root is drunk as an anthelmintic. In Tanzania a root decoction is taken to treat back pain, hernia, and dizziness, a root infusion is drunk as an aphrodisiac, and leaf decoctions are drunk for treating fungal infections and convulsions . In parts of East Africa the roots, bark, and leaves are boiled and the vapor is inhaled for the treatment of influenza and fever. In Namibia a root or bark decoction is drunk by women for the treatment of postnatal and stomach pains, a leaf infusion is drunk for treating cough and chest complaints, and a fruit decoction is drunk to relieve pain during pregnancy and after giving birth. In Malawi the irritant hairs along the splitting point of the fruits are reported to be burnt and the ash is used in an ointment for the treatment of eye infections, especially in babies .
Acacia sieberiana has been utilized traditionally for treatment of skin eruptions and rheumatic pains and in treatment of syphilis, gastritis, cough, fever, ringworm, leprosy, epilepsy, dysentery, and mouth ulcers, as a vermicide and contraceptive . Communities in South Africa and Ethiopia traditionally utilize Acacia sieberiana for the treatment of various ailments including inflammation, tiredness, joint pains, bilharzia, and fever and as an enema [28, 29]. The stem and root bark extract, both rich in tannins, are used in treating schistosomiasis, fever, stomach ache, jaundice, cough, sexual impotence, erectile dysfunction, haemorrhoids, syphilis, and uterine problems and to improve lactation after child birth . Laboratory studies on Acacia sieberiana reveal that it is effective against Bacillus subtilis, Staphylococcus epidermidis, Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae, and Mycobacterium avium. Preliminary phytochemical screening showed presence of saponins, tannins, cardiac glycosides, flavonoids, and anthraquinones [31, 32]. The crushed pods are used in the treatment of hypertension in the Blue Nile state in Sudan .
This study was undertaken to establish the antibacterial activity of Cassia abbreviata ssp. abbreviata, Acacia sieberiana, and Sterculia africana against Gram positive and Gram negative bacteria of medical and veterinary concern. Results observed may contribute to knowledge on the range of microbes susceptible to these plant extracts using a single solvent.
Furthermore, results from this study may shed more light on the existing knowledge gap about the therapeutic benefits of plant metabolites present in the nonhuman primates’ diet. A combined understanding of the underlying wildlife behaviour with long-term dietary data, antimicrobial studies, and analysis of plant chemistry will contribute to better understanding of the animal self-medication theory.
2. Materials and Methods
2.1. Plant Collection and Processing
Plant materials were collected from areas around Sokoine University of Agriculture main campus and areas around Mikumi National Park in Morogoro region, Tanzania, the location of which is shown in Figure 1. The samples were collected in the month of September during a dry spell when Sterculia africana had shed its leaves and therefore these were not studied.
The samples were collected during morning hours and authenticated with the help of Mr. Frank Mbago, a Botanist from the University of Dar es Salaam. They were assigned voucher specimen numbers FMM 3704, 3705, and 3706 for Acacia sieberiana, Sterculia africana, and Cassia abbreviata ssp. abbreviata, respectively. From Sterculia africana, the root bark and stem bark were collected while Cassia abbreviata ssp. abbreviata and Acacia sieberiana samples consisted of the stem bark, root bark, and leaves. The 8 samples were cleaned; the stem bark and root bark were cut into smaller pieces to allow for better drying in air. After drying, the samples were pulverised to a particle size of 1 mm.
2.2. Extraction of Samples
In the chemistry laboratory at Mazimbu Campus, Sokoine University of Agriculture, Tanzania, one sample was handled at a time in order to avoid cross contamination. Using a Yanhe Analytical Electronic Balance, the dry samples each were weighed into a separate clean, marked plastic container of known weight and the sample weight was recorded. The menstruum used was ethanol (Sigma-Aldrich) of analytical grade which was added till all the sample was fully soaked and macerated for 3 days with constant shaking. The samples were then strained and filtered using 110 mm Whatman filter paper and excess menstruum was evaporated using a rotary evaporator and then finally transferred to dry in an oven (Shel Lab) till constant weight. The marc was resoaked in fresh ethanol for 3 days and treated as the previous samples and then dried before being added to the corresponding samples. The crude plant extracts were then coded as shown in Table 1 and stored at room temperature before use for subsequent analysis.
2.3. Antibacterial Screening
2.3.1. Test Microorganisms
Agar well diffusion method was used for preliminary analysis to screen for samples with antibacterial activity and thereafter MIC was used for analysis.
Bacterial strains used in this study were standardized by the American Type Cell Collection (ATCC/Manassas, VA/USA) and constituted of Gram negative and Gram positive bacteria. The Gram negative bacteria tested were Salmonella paratyphi (ATCC 9150), Klebsiella pneumoniae (ATCC 13883), Shigella sonnei (ATCC 25931), Enterobacter cloacae (ATCC 23355), Pseudomonas aeruginosa (ATCC 27853), and Escherichia coli (ATCC 25922). Gram positive bacteria tested were Enterococcus faecalis (ATCC 51299) and Staphylococcus aureus (ATCC 25923). Bacterial culture cells were maintained at 37°C on Muller-Hinton (MH) agar on slants until required. All microbial species used were supplied by the Department of Microbiology, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania.
2.3.2. Minimum Inhibitory Concentration (MIC)
The Minimum Inhibitory Concentration (MIC) is the lowest concentration of crude plant extract that inhibits growth of test microorganisms. A modified method by  was employed in determining the MIC of the different sample extracts. Sterile 100 μl of Muller-Hinton broth was measured in each well of a 96-well microtiter plate . 100 μl of 10 mg/ml of extract prepared in dimethyl sulfoxide (DMSO) was added to row 1 and mixed using a micropipette. This was followed by serial dilution to row 8 with the additional 100 μl therein discarded. One column was used for sterility control (no culture was added), another column was used as a negative control (100 μl of DMSO without extract), and another column was used as the positive control with Gentamicin (0.01 mg/ml) making a total of 12 rows. 0.5 McFarland standard suspension of test bacteria was made in nutrient broth from which 100 μl of the final inoculums containing approximately 1 × 108 cfu/ml was added to each well except the sterility control to make a final volume of 200 μl. The experiments were performed in duplicate. The microtiter plates were sealed in a plastic film and then incubated at 37°C in a humidified incubator for 18 hrs. After incubation, 40 μl of 0.2 mg/ml iodonitrotetrazolium (INT) was added to each well and the microtiter plates were incubated for another 2 hours before removal for observation. The development of a purple colour resulting from the formation of the red/purple formazan was an indication of growth (positive indicator of cell viability). Decrease in the intensity of the red/purple formazan colour was indicative of inhibition of growth of test microorganisms.
The MIC results tabulated in Table 2 showed that the crude plant extracts were active against all the test microorganisms. The lowest MIC value was 0.16 mg/ml as compared with 0.01 mg/ml of the standard, Gentamicin. The negative control showed development of a purple colour resulting from formation of the purple formazan which is a positive indicator of cell viability whereas the sterility control showed no colour change, an indication of absence of test microorganisms. Minimum Inhibitory Concentration ranged from 0.16 mg/ml to 2.5 mg/ml with 0.16 mg/ml being the lowest value denoting the greatest antibacterial efficacy as presented by A.R and A.S plant parts. Gram negative bacteria in the ethanol extracts had higher MIC values as opposed to the Gram positive bacteria. A.R and A.S demonstrated the lowest MIC values of 0.16 mg/ml and 0.31 mg/ml for both Pseudomonas aeruginosa and Klebsiella pneumoniae strains. The lowest MIC value for Gram positive strains was 0.31 mg/ml demonstrated by B.R and B.L against Staphylococcus aureus. Overall, plants A and B presented lower MIC values as opposed to plant C. The experiments were performed in duplicate and the average value was tabulated in Table 2.
Cassia abbreviata ssp. abbreviata, Acacia sieberiana, and Sterculia africana extracts possess antibacterial activity against the test strains as shown from the results of the in vitro experiment in Table 2. Cassia abbreviata ssp. abbreviata and Acacia sieberiana exhibited better activity against the test microorganisms as compared to Sterculia africana with Cassia abbreviata ssp. abbreviata showing the lowest MIC value of 0.16 mg/ml of the three plants. The positive control was a standard drug, Gentamicin (0.01 mg/ml), an aminoglycoside targeting the bacterial ribosome. Interestingly, the lowest crude plant extract MIC of 0.16 mg/ml was only 16-fold weaker than Gentamicin. The crude plant extracts performed better than the negative control to a concentration of 0.313 mg/ml of extract. Other studies that reported on Cassia abbreviata ssp. abbreviata and Acacia sieberiana concur with these findings [16, 17, 30]. A viable challenge in interpreting self-medication is differentiating between plants that are ingested for their nutritional value but are medicinal foods and therefore offer passive prevention and plants ingested solely for their therapeutic medicinal use, medicinal plants. This challenge exists in traditional human societies where medicine and food are often of the same origin [2, 3, 28, 34]. For instance, traditional spices and condiments of daily Asian cuisine such as marine algae, ginger root, turmeric and herbs also play an important role in suppressing viral and parasite infections [2, 34, 35]. Results showed that different concentrations of the plant extracts were required to inhibit the growth of different microbes due to the difference in potency of the plant extracts attributed to the phytochemicals present, environment of growth, or extraction method used. The variation in the susceptibility of microorganisms could also be attributed to their intrinsic properties that are related to the permeability of their cell surface to the plants extracts. Their pharmacological effect could therefore be experienced with repeated ingestion or work together in synergy.
This study provides evidence that the yellow baboon forages for similar plants also used in ethno medicine as seen from previous literature reviewed [16, 17, 22, 23]. 27.5% of microbes that affect nonhuman primates affect humans as well, Staphylococcus aureus, Salmonella, and Escherichia coli being only a few examples of such microbes, a factor attributed to our phylogenetic closeness [4, 12, 36, 37]. It should therefore not come as a surprise that human and nonhuman primates perhaps select similar plants when challenged with similar illnesses. It is known that nonhuman primates feed on a great variety of plant species; however it is prudent to take note of the ones that are used as feed infrequently and/or in isolated cases in an uncommon manner as was the case with the yellow baboons in Mikumi National Park. It may not be possible to pharmacologically analyse or carry out phytochemical screening for each plant; however, special attention should be paid to plants used as feed for nonhuman primates exhibiting potential symptoms of illness or in uncommon feeding behaviour [6–9, 11, 38] including but not limited to a lack of appetite, intestinal disorder, coughing, increased parasite load, adult parasite worm expulsion in the dung, and discoloration of urine.
The results of the in vitro experiment in Table 2 demonstrate that ethanol extracts from the different plant parts of Cassia abbreviata ssp. abbreviata, Acacia sieberiana, and Sterculia africana exhibit antibacterial activity against the test microbes. Some of these microorganisms are of medical and veterinary importance because they affect both humans and nonhuman primates. Additionally, these findings contribute to the foraging theory that suggests that the diet of nonhuman primates contains plants that are used as feed for self-medication. Their diet could therefore act as a sieve through which plants used as feed in an uncommon manner by nonhuman primates are tested. A combination of long-term dietary data, pharmacological studies, and analysis of plant chemistry may lead to the discovery of new therapeutic leads and also offer a better understanding of self-medication in animals. In addition to that, conservation of the plants used as feed is vital for primate conservation due to the pharmacological benefits obtained from these medicinal trees. These findings therefore propose the study of animal self-medication (zoopharmacognosy) as an alternative field in search for new therapeutic leads and drug discovery and further suggest that nonhuman primates feed on the nonnutritive parts of these plants for their therapeutic benefits.
Conflicts of Interest
The authors declare that there are no conflicts of interest regarding the publication of this work.
The authors acknowledge INTRA-ACP mobility scholarship and RISE-AFNNET for partially funding this research and the Faculty of Veterinary Medicine, Sokoine University of Agriculture, for facilitating this study. They thank in a special way Mr. Mwesongo James, Mr. Frank Mbago, and Ms. Anna Mpanyakavili for their invaluable help. Safari Bookings is also acknowledged for the map of Mikumi National Park.
V. Carrai, S. M. Borgognini-Tarli, M. A. Huffman, and M. Bardi, “Increase in tannin consumption by sifaka (Propithecus verreauxi verreauxi) females during the birth season: A case for self-medication in prosimians?” Primates, vol. 44, no. 1, pp. 61–66, 2003.View at: Google Scholar
M. A. Huffman, “Current evidence for self-medication in primates: a multidisciplinary perspective,” Yearbook of Physical Anthropology, vol. 40, pp. 171–200, 1997.View at: Google Scholar
K. Koshimizu, H. Ohigashi, M. A. Huffman, T. Nishida, and H. Takasaki, “Physiological activities and the active constituents of potentially medicinal plants used by wild chimpanzees of the Mahale mountains, Tanzania,” International Journal of Primatology, vol. 14, no. 2, pp. 345–356, 1993.View at: Publisher Site | Google Scholar
M. A. Huffman, S. Gotoh, D. Izutsu, K. Koshimizu, and M. S. Kalunde, “Further observations on the use of the medicinal plant, Vernonia amygdalina (Del) by a wild chimpanzee, its possible effect on parasite load, and its phytochemistry,” African Study Monographs, vol. 14, no. 4, pp. 227–240, 1993.View at: Google Scholar
M. A. Huffman, “Control of nematode infections by African great apes: a new paradigm for treating parasite infection with natural medicines?” American Association of Veterinary Parasitologists, vol. 20, no. 2, pp. 3–7, 1998.View at: Google Scholar
E. K. Glander, Eating on the Wild Side, The University of Arizona Press, London, UK, 1994.
D. Cousins and M. A. Huffman, “Medicinal properties in the diet of gorillas: an Ethno-pharmacological evaluation,” African Study Monographs, vol. 23, no. 2, pp. 65–89, 2002.View at: Google Scholar
E. Rodrigues, M. L. Petroni, and M. Huffman, “Medicinal plants in the diet of woolly spider monkeys (Brachyteles arachnoides,E. Geoffroy,1806)- a bio-rational for the search of new medicines for human use?” Brazilian Journal of Pharmacognosy, vol. 27, pp. 135–142, 2016.View at: Google Scholar
N. I. Mongalo and B. J. Mafoko, “Cassia abbreviata Oliv. A review of its ethnomedicinal uses, toxicology, phytochemistry, possible propagation techniques and Pharmacology,” African Journal of Pharmacy and Pharmacology, vol. 7, no. 45, pp. 2901–2906, 2013.View at: Google Scholar
M. Mwila and P. Shiv, “Antimicrobial activity and potency of cassia abbreviataoliv stem bark extracts,” International Journal of Pharmacy and Pharmaceutical Sciences, vol. 6, pp. 426–428, 7.View at: Google Scholar
J. N. Otieno, J. J. Magadula, E. Kakudidi, C. Kirimhuzya, J. Orodho, and P. Okemo, “Use of ethnobotanical criteria for conservation assessment of plants used for respiratory diseases in Lake Victoria region, Tanzania,” International Journal of Biodiversity and Conservation, vol. 3, no. 11, pp. 610–617, 2011.View at: Google Scholar
H. H. Gibreel, M. A. Y. Kordofani, E. I. Warrag, and H. O. Ahmed, “Medicinal value and ecotaxonomy of the flora of Blue Nile State-Sudan,” Journal of Chemical and Pharmaceutical Research, vol. 5, no. 2, pp. 36–43, 2013.View at: Google Scholar
J. M. Mainen, J. P. Carolien, B. Vanden, and J. M. Omar, “Brine shrimp toxicity evaluation of some Tanzanian plants used traditionally for the treatment of fungal infections,” African Journal of Traditional Complementary and Alternative Medicine, vol. 2, pp. 219–225, 2007.View at: Google Scholar
W. Obidah, U. A. Saad, and A. U. Wurocheke, “Toxic effects of aqueous stem bark of cassia sieberiana on some biochemical parameters in rats,” African Journal of Biochemistry Research, vol. 3, no. 5, pp. 229–231, 2009.View at: Google Scholar
I. G. Doka and S. M. Yagi, “Ethnobotanical survey of medicinal plants in West Kordofan (Western Sudan),” Ethnobotanical Leaflet, vol. 13, pp. 1409–1416, 2009.View at: Google Scholar
J. D. Christiana, L. E. Ishaku, V. O. Nkechi et al., “Antidiarrheal evaluation of aqueous and ethanolic leaf extracts of acacia sieberiana DC. (Fabaceae) in Albino Rats,” Asian Journal of Experimental Biological Sciences, vol. 3, no. 4, pp. 779–803, 2012.View at: Google Scholar
H. M. Burkill, “The useful plants of west tropical Africa,” Royal Botanic Garden, vol. 3, pp. 199–201, 1995.View at: Google Scholar
H. Mahdi, K. Palmina, and C. Tony, “Analysis of commercial vegetable tannin materials and related polyphenols of selected acacia species,” Journal of Forest Products and Industry, vol. 2, no. 1, pp. 21–28, 2013.View at: Google Scholar
C. Vijayameena, G. Submachine, M. Loganayagi, and B. Ramesh, “Phytochemical screening and assessment of antibacterial activity for the bioactive compounds in Annona muricata,” International Journal of Current Microbiology Applied Science, vol. 2, no. 1, pp. 1–8, 2013.View at: Google Scholar