Combined Effect of Methanol Extracts and Essential Oils of Callistemon rigidus (Myrtaceae) and Eucalyptus camaldulensis (Myrtaceae) against Anopheles gambiae Giles larvae (Diptera: Culicidae)

Table 3

Mortality percent of A. gambiae larvae treated with the combination of methanol extracts of E. camaldulensis and C. rigidus and LC₅₀ as well as LC₉₅ (ppm) values 24 h postexposure.

Combinations	Conc (ppm)	% mortality	Slope ± SE	LC50 (LFL-UFL) (ppm)	LC95 (LFL-UFL) (ppm)	χ^2
E100%	0	0.00 ± 0.00D	5.18 ± 0.22	408.90 (384.79–434.53)	849.50 (769.31–959.86)	34.93
	125	0.00 ± 0.00D
	250	15.00 ± 3.00C
	500	66.00 ± 5.29B
	1000	98.00 ± 1.15A
	Bi-one	100 ± 0.00A
	F(5,18)	350.96
E75% : C25%	0	0.00 ± 0.00D	2.93 ± 0.17	117.91 (81.28–147.90)	426.97 (329.72–673.50)	133.33
	125	47.00 ± 2.51C
	250	91.00 ± 2.51B
	500	97.00 ± 1.91AB
	1000	98.00 ± 1.15AB
	Bi-one	100 ± 0.00A
	F(5,18)	560.70
E50% : C50%	0	0.00 ± 0.00D	4.94 ± 0.23	183.37 (167.85–199.15)	394.28 (346.73–469.01)	57.66
	125	17.00 ± 1.91C
	250	92.00 ± 2.58B
	500	96.00 ± 1.63A
	1000	100 ± 0.00A
	Bi-one	100 ± 0.00A
	F	943.83
E25% : C75%	0	0.00 ± 0.00D	3.46 ± 0.24	106.21 (82.49–124.98)	317.17 (265.39–422.31)	65.63
	125	58.00 ± 2.58C
	250	93.00 ± 1.91B
	500	98.00 ± 2.00AB
	1000	100 ± 0.00A
	Bi-one	100 ± 0.00A
	F(5,18)	671.17
C100%	0	0.00 ± 0.00D	1.80 ± 0.18	39.15 (24.95–53.24)	319.80 (278.04–380.77)	16.67^ns
	125	83.00 ± 1.91C
	250	91.00 ± 1.00B
	500	98.00 ± 1.15A
	1000	100 ± 0.00A
	Bi-one	100 ± 0.00A
	F(5,18)	1528.66

Mean of mortality ± standard deviation within a column followed by the same letter did not differ significantly according to Tukey test (); ; ; ; LC = lethal concentration; LFL: lower fiducial limit; UFL: upper fiducial limit; Bi-one = positive control tested at 1000 ppm; number of replicates: 4. E100%, 75%, 50%, 25%, and 0% = E. camaldulensis 100%, 75%, 50%, 25%, and 0%, respectively; C100%, 75%, 50%, 25%, and 0% = C. rigidus 100%, 75%, 50%, 25%, and 0%, respectively.