Combined Effect of Methanol Extracts and Essential Oils of Callistemon rigidus (Myrtaceae) and Eucalyptus camaldulensis (Myrtaceae) against Anopheles gambiae Giles larvae (Diptera: Culicidae)

Table 5

Mortality percent of A. gambiae larvae treated with the combination of the essential oils of E. camaldulensis and C. rigidus and LC₅₀ as well as LC₉₅ (ppm) values 24 h postexposure.

Combinations	Conc (ppm)	% mortality	Slope ± SE	LC50 (LFL-UFL) (ppm)	LC95 (LFL-UFL) (ppm)	χ^2
E100%	0	0.00 ± 0.00C	7.35 ± 0.40	223.03 (213.51–233.14)	337.17 (345.86–410.84)	24.54^ns
	25	0.00 ± 0.00C
	50	0.00 ± 0.00C
	100	0.00 ± 0.00C
	200	31.00 ± 3.00B
	Bi-one	100.0 ± 0.00A
	F(5,18)	1729
E75% : C25%	0	0.00 ± 0.00D	3.40 ± 0.13	62.87 (52.48–74.23)	191.03 (149.12–278.02)	165.60
	25	0.00 ± 0.00D
	50	50.00 ± 4.16C
	100	81.00 ± 2.51B
	200	89.00 ± 3.41B
	Bi-one	100.0 ± 0.00A
	F(5,18)	389.02
E50% : C50%	0	0.00 ± 0.00D	2.42 ± 0.09	132.58 (102.77–176.29)	632.22 (397.71–1460.60)	256.36
	25	0.00 ± 0.00D
	50	29.00 ± 4.43C
	100	38.00 ± 2.58BC
	200	43.00 ± 1.91B
	Bi-one	100.0 ± 0.00A
	F(5,18)	404.65
E25% : C75%	0	0.00 ± 0.00E	3.07 ± 0.11	115.16 (101.71–130.69)	394.96 (318.00–528.64)	84.48
	25	0.00 ± 0.00E
	50	20.00 ± 1.63D
	100	43.00 ± 3.41C
	200	66.00 ± 2.58B
	Bi-one	100.0 ± 0.00A
	F(5,18)	691.00
C100%	0	0.00 ± 0.00E	3.73 ± 0.14	99.66 (89.32–111.32)	274.76 (229.71–348.72)	81.32
	25	0.00 ± 0.00E
	50	21.00 ± 3.41D
	100	40.00 ± 3.65C
	200	88.00 ± 3.65B
	Bi-one	100.0 ± 0.00A
	F(5,18)	376.93

Mean of mortality ± standard deviation within a column followed by the same letter did not differ significantly according to Tukey test (); ; ; LC: lethal concentration; LFL: lower fiducial limit; UFL: upper fiducial limit; Bi-one = positive control tested at 1000 ppm; number of replicates: 4. E100%, 75%, 50%, 25%, and 0% = E. camaldulensis 100%, 75%, 50%, 25%, and 0%, respectively; C100%, 75%, 50%, 25%, and 0% = C. rigidus 100%, 75%, 50%, 25%, and 0%, respectively.