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Interdisciplinary Perspectives on Infectious Diseases
Volume 2008 (2008), Article ID 314762, 10 pages
Research Article

Combining Microarray Technology and Molecular Epidemiology to Identify Genes Associated with Invasive Group B Streptococcus

1Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, MI 48109, USA
2Program in Bioinformatics, Eastern Michigan University, Ypsilanti, MI 48197, USA
3Fargo VA Medical Center, Fargo, ND 58102, USA

Received 29 August 2007; Accepted 29 November 2007

Academic Editor: Joshua P. Metlay

Copyright © 2008 Lixin Zhang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

NimbleGen uses custom software to identify potential different probe sequences in the tester strains. Briefly, the average reference/tester ratio of both forward and reverse sequence probes was calculated to identify a difference in a probe sequence between tester and reference strains. A threshold for a difference callâ was established based on the signals within 1800 bp of the current position. The tester probe sequence is classified to be different from reference if its average signal ratio is greater than the threshold. This process is iterated for all base pairs in the genome to calculate local thresholds for all probe sequences.

  1. Supplementary Text