Sterol Biosynthesis Pathway as Target for Anti-trypanosomatid Drugs
Figure 12
DIC microscopy (left panel) and immunofluorescence microcospy (right panel) of L. amazonensis promastigotes control (a)-(b) and treated with ER-119884 (c)-(d). The labeling corresponds to the cytoskeleton constituted mainly by tubulin, revelead here by using of an Alexa 488-labeled secondary antibody. The black arrow in DIC image corresponds to the cell body which sometimes appeared changed and rounded as compared to the control parasites, while the white arrows point to several tubulin clusters that accumulated in the cytosol after treatment. All images are reproduced with permission from [37] American Society for Microbiology. Bars, m.
