Towards an Understanding of the Herpes Simplex Virus Type 1 Latency-Reactivation Cycle

Figure 3

Schematic of factors encoded within the LAT locus. (a) Schematic of genes within the long repeats that contain the LAT locus. The large arrow indicates the primary LAT transcript. The solid rectangle represents the very stable 2 kb LAT intron. The start of LAT transcription is indicated by the arrow at +1 (genomic nucleotide 118801). Several restriction enzyme sites and the relative locations of the ICP0 and ICP34.5 transcripts are shown for reference. The locations of the 6 micro-RNAs (miR-H1-6) that are located within the 8.3 kb LAT [136] are shown. (b) Partial restriction map of LAT and position of LAT open reading frames (L1-8) within the first 1.5 Kb of strain McKrae LAT coding sequences, which were based on previous studies [137]. The numbering system of the ORFs was consistent with a previous study [137]. Only the ORFs with at least 30 amino acids are shown (the number of amino acids in each ORF is denoted by the numbers in brackets). Open circles denote the position of two LAT small RNAs that are encoded within the first 1.5 kb LAT coding sequences [138]. Positions of UOL transcript, AL transcript, and ORFs located on the opposite strand of LAT (AL2 and AL3) are shown. The numbers of amino acids of AL2 and AL3 are in brackets. Nucleotide positions relative to the start of LAT transcription are not shown in parenthesis. Numbers in parentheses represent HSV-1 nucleotide positions.