Quantitative Evaluation of Bacteria Adherent and in Biofilm on Single-Wall Carbon Nanotube-Coated Surfaces
Figure 1
Color switch of BTA reagents and BTA correlation lines, (a)-(b) optical absorbance expressed as optical density versus the wave length in the visible region for BT-PR (a) and BT-RZ (b) reagents. Solid line: optical absorbance of BTA reagent before the switch; dotted line: optical absorbance of BTA reagent after the switch. Inserts: (a) BT-PR reagent color before (left) and after (right) the switch (red and yellow, resp.); (b) BT-RZ reagent color before (left) and after (right) the switch (blue and pink, resp.). (c)-(d) correlation lines correlate the time (hours) for color switch of BT-PR (c) and BT-RZ (d) reagents and log of the initial number N0 of planktonic Streptococcus mutans ATCC 25175T (c) and Pseudomonas aeruginosa ATCC 15692 (d). Correlation lines were described by the following linear equations: y = −0.21 32x + 7.9597 and = 0.9899 for S. mutans (c) and y = −0.4675x + 8.5421 and = 0.9968 for P. aeruginosa (d).
