Identification of Sphingomyelinase on the Surface of Chlamydia pneumoniae: Possible Role in the Entry into Its Host Cells
Figure 7
Effect of heat treatment on the SMase activity of C. pneumoniae. Bacteria were incubated for 5 min at either 60 (■) or 90°C (●), and bacteria maintained at 37°C were used as a control (▲). Amplex Red assays were started by adding 1 × 107 IFUs of bacteria and incubated in a total volume of 200 μL at 37°C. Resorufin fluorescence FI a.u. (fluorescence intensity, arbitrary units) was measured.
