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Interdisciplinary Perspectives on Infectious Diseases
Volume 2014, Article ID 607287, 6 pages
http://dx.doi.org/10.1155/2014/607287
Research Article

ITS1 PCR-RFLP Diagnosis and Characterization of Leishmania in Clinical Samples and Strains from Cases of Human Cutaneous Leishmaniasis in States of the Mexican Southeast

1Escuela Nacional de Ciencias Biológicas, IPN, Carpio y Plan de Ayala, 11340 Mexico City, Mexico
2School of Medicine Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University of Jerusalem, 9112102 Jerusalem, Israel
3Centro de Investigaciones Biomédicas, Universidad Autonoma de Campeche, Patricio Trueba, 24090 Campeche, Mexico
4Instituto de Diagnóstico y Referencia Epidemiológicos, Francisco P. de Miranda Lomas de Plateros, 01480 Mexico City, Mexico

Received 10 January 2014; Accepted 9 June 2014; Published 1 July 2014

Academic Editor: Stéphane Picot

Copyright © 2014 Amalia Monroy-Ostria et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

American cutaneous leishmaniasis includes a spectrum of clinical forms localized cutaneous, diffuse cutaneous, and mucocutaneous leishmaniasis which can be caused by different strains of Leishmania belonging to the L. mexicana or L. braziliensis complexes which may coexist in the same endemic area. We evaluated the PCR-RFLP assay of the ITS1 genes for direct identification of Leishmania species in 163 clinical samples and 21 Mexican isolates of Leishmania. In relation to the Mexican isolates of Leishmania 52% displayed a pattern similar to the L. (L.) mexicana, 5% showed a mixed pattern compatible with L. (L.) mexicana and L. (V.) braziliensis, eight with L. (L.) amazonensis and L. (L.) mexicana, and one to L. (V.) braziliensis. Most of the clinical samples, 109/116 (94%), gave a pattern similar to that of the L. mexicana, two clinical samples gave similar patterns to that of Leishmania braziliensis, and 5 samples gave patterns that suggest a coinfection of L. (L.) mexicana and L. (V.) braziliensis or L. (L.) mexicana and L. (L.) amazonensis. The ITS1 PCR-RFLP assay is a multipurpose tool for diagnosis of Leishmania from clinical samples and enables determination of the infecting species of New World Leishmania in the field in relatively short time and low cost.