Lowly Expressed Ribosomal Protein S19 in the Feces of Patients with Colorectal Cancer

Figure 4

Efficiency of RPS19 silence in colonic cells by RNA interference. RPS19 silence is achieved by the lentivirus-mediated RNAi experiment. Relative mRNA levels of RPS19 are quantified by qRT-PCR with TaqMan probes and normalized by individual level of 18 s rRNA. The relative expression level of shLuc-infected cells is considered as 1. Results are representative of those obtained in two-to-three separate experiments with error bars showing standard error. Changes of protein levels are immunoblotted with antibodies against RPS19 and β-actin (in black square).