Figure 4: DKK1 inhibits Wnt3a-mediated β-catenin translocation. (A) Immunostaining of β-catenin in L-cells following stimulation with Wnt3a CM, either without DKK1 or in the presence of increasing volumes of DKK1 CM. Panel (a), unstimulated cells; panel (b), Wnt3a-stimulated cells; panels (c–f), Wnt3a-stimulated cells in presence of DKK1 CM dilutions. Cell nuclei counterstaining with Hoechst 33342 dye are shown for each panel. (B) Histogram representation of the nuclear/cytoplasmic intensity ratios corresponding to the cells shown in (A).