Genotyping of CYP2D6 Polymorphisms by MALDI-TOF Mass Spectrometry in Sardinian People

Table 3

Sequences and molecular weights of unextended (UEP) and extended (EP) iPLEX primers.

	UEPs’ sequences	UD	UM	EP lower mass sequences	ELM	ELC	EP higher mass sequences	EHM	EHC
(1) 13-PLEX
3877G>A	CCTTCCGCTTCCACCCC	F	4978	CCTTCCGCTTCCACCCCA	5249	A	CCTTCCGCTTCCACCCCG	5265	G
3183G>A	CCGCACCTGCCCTATCA	R	5051	CCGCACCTGCCCTATCAC	5299	G	CCGCACCTGCCCTATCAT	5378	A
2615_2617  delAAG	GGCAGCCACTCTCACCT	R	5091	GGCAGCCACTCTCACCTC	5339	del	GGCAGCCACTCTCACCTT	5418	AGA
2483G>T	GCGTAGGACCTTGCCAG	R	5211	GCGTAGGACCTTGCCAGC	5459	G	GCGTAGGACCTTGCCAGA	5483	T
3853G>A	GAAGCGGAAGGGCTTCT	R	5275	GAAGCGGAAGGGCTTCTC	5523	G	GAAGCGGAAGGGCTTCTT	5603	A
3277T>C	TGCAGCGCTTTGGGGACA	F	5556	TGCAGCGCTTTGGGGACAC	5803	C	TGCAGCGCTTTGGGGACAT	5883	T
4180G>C	AGCTCATAGGGGGATGGG	R	5645	AGCTCATAGGGGGATGGGC	5892	G	AGCTCATAGGGGGATGGGG	5932	C
2939G>A	TGGGCTCACGCTGCACATC	R	5766	TGGGCTCACGCTGCACATCC	6013	G	TGGGCTCACGCTGCACATCT	6093	A
3288G>A	GATGTCATATGGGTCACAC	R	5828	GATGTCATATGGGTCACACC	6075	G	GATGTCATATGGGTCACACT	6155	A
3176C>T	GGCCGTGTCCAACAGGAGAT	F	6167	GGCCGTGTCCAACAGGAGATC	6414	C	GGCCGTGTCCAACAGGAGATT	6494	T
2850C>T	AGCTTCAATGATGAGAACCTG	F	6454	AGCTTCAATGATGAGAACCTGC	6701	C	AGCTTCAATGATGAGAACCTGT	6781	T
2935A>C	AGTGCTCACGCTGCACATCCGGA	R	7010	AGTGCTCACGCTGCACATCCGGAG	7297	C	AGTGCTCACGCTGCACATCCGGAT	7337	A
1757C>T	TTGTGCCGCCTTCGCCAACCACTC	F	7201	TTGTGCCGCCTTCGCCAACCACTCC	7448	C	TTGTGCCGCCTTCGCCAACCACTCT	7528	T
(2) 14-PLEX
1661G>C	CAGAGGCGCTTCTCCGT	F	5162	CAGAGGCGCTTCTCCGTC	5410	C	CAGAGGCGCTTCTCCGTG	5450	G
1869T>C	ACGGCTTTGTCCAAGAG	R	5210	ACGGCTTTGTCCAAGAGA	5482	T	ACGGCTTTGTCCAAGAGG	5498	C
1846G>A	GGGGCGAAAGGGGCGTC	R	5342	GGGGCGAAAGGGGCGTCC	5588	G	GGGGCGAAAGGGGCGTCT	5668	A
214G>C	TGGAGGGCGGCAGAGGT	F	5357	TGGAGGGCGGCAGAGGTC	5604	C	TGGAGGGCGGCAGAGGTG	5644	G
19G>A	CTGGGGCTAGAAGCACTG	F	5565	CTGGGGCTAGAAGCACTGA	5836	A	CTGGGGCTAGAAGCACTGG	5852	G
1707delT	GCGGCCTCCTCGGTCACCC	R	5702	GCGGCCTCCTCGGTCACCCC	5949	del	GCGGCCTCCTCGGTCACCCA	5973	T
746C>G	GCGACCCAGCCTCCTGATC	R	5710	GCGACCCAGCCTCCTGATCC	5957	G	GCGACCCAGCCTCCTGATCG	5997	C
997C>G	GAACAGGCGGGCGGTCGGC	R	5920	GAACAGGCGGGCGGTCGGCC	6167	G	GAACAGGCGGGCGGTCGGCG	6207	C
100C>T	AATGCTGGGCTGCACGCTAC	F	6118	AATGCTGGGCTGCACGCTACC	6365	C	AATGCTGGGCTGCACGCTACT	6445	T
1979T>C	GGACAGCCCGACTCCTCCTTC	R	6303	GGACAGCCCGACTCCTCCTTCA	6574	T	GGACAGCCCGACTCCTCCTTCG	6590	C
-948C>A	CAGGCTGGGGCAAGGGCCTTC	F	6488	CAGGCTGGGGCAAGGGCCTTCC	6735	C	CAGGCTGGGGCAAGGGCCTTCA	6759	A
1749A>G	GCCCATCACCCACCGGAGTGGT	R	6681	GCCCATCACCCACCGGAGTGGTC	6929	G	GCCCATCACCCACCGGAGTGGTT	7008	A
77G>A	GTAGCGTGCAGCCCAGCGTTGG	R	6792	GTAGCGTGCAGCCCAGCGTTGGC	7040	G	GTAGCGTGCAGCCCAGCGTTGGT	7120	A
1513C>T	AGCTGGACAGAGCCAGGGACTG	F	6835	AGCTGGACAGAGCCAGGGACTGC	7082	C	AGCTGGACAGAGCCAGGGACTGT	7162	T
(3) 13-PLEX
984A>G	GCGAGTGTCCTCGCCG	R	4874	GCGAGTGTCCTCGCCGC	5121	G	GCGAGTGTCCTCGCCGT	5201	A
1863_1864ins  TTTCGCCCC	GACGCCCCTTTCGCCCC	F	5043	GACGCCCCTTTCGCCCCA	5315	–	GACGCCCCTTTCGCCCCT	5370	TTTCGCCCC
1039C>T	ACCCAGATCCTGGGTTT	F	5161	ACCCAGATCCTGGGTTTC	5409	C	ACCCAGATCCTGGGTTTT	5489	T
1943G>A	CCAGCAGCCTGAGGAAG	R	5229	CCAGCAGCCTGAGGAAGC	5477	G	CCAGCAGCCTGAGGAAGT	5557	A
974C>A	CGGCCGTGCGCGAGGCG	F	5253	CGGCCGTGCGCGAGGCGC	5501	C	CGGCCGTGCGCGAGGCGA	5525	A
1704C>G	GGCAAGAAGTCGCTGGAG	F	5614	GGCAAGAAGTCGCTGGAGC	5861	C	GGCAAGAAGTCGCTGGAGG	5901	G
310G>T	TAAATGCCCTTCTCCAGGA	R	5748	TAAATGCCCTTCTCCAGGAC	5995	G	TAAATGCCCTTCTCCAGGAA	6019	T
957C>T	GTCGTGCTCAATGGGCTGG	F	5876	GTCGTGCTCAATGGGCTGGC	6123	C	GTCGTGCTCAATGGGCTGGT	6203	T
883G>C	TCCCCGAAGCGGCGCCGCAA	R	6073	TCCCCGAAGCGGCGCCGCAAC	6320	G	TCCCCGAAGCGGCGCCGCAAG	6360	C
1659G>A	CGCGAGCAGAGGCGCTTCTCC	F	6408	CGCGAGCAGAGGCGCTTCTCCA	6679	A	CGCGAGCAGAGGCGCTTCTCCG	6695	G
31G>A	AGCAGGAAGATGGCCACTATCA	R	6777	AGCAGGAAGATGGCCACTATCAC	7024	G	AGCAGGAAGATGGCCACTATCAT	7104	A
1724C>T	GCGAAGGCGGCACAAAGGCAGGC	R	7158	GCGAAGGCGGCACAAAGGCAGGCA	7429	T	GCGAAGGCGGCACAAAGGCAGGCG	7445	C
124G>A	TCACATGCAGCAGGTTGCCCAGCC	R	7299	TCACATGCAGCAGGTTGCCCAGCCC	7546	G	TCACATGCAGCAGGTTGCCCAGCCT	7626	A
(4) 14-PLEX
3198C>G	ACCCATCTCTGGTCGCC	R	5082	ACCCATCTCTGGTCGCCC	5330	G	ACCCATCTCTGGTCGCCG	5370	C
2549delA	TGGGTCCCAGGTCATCC	R	5162	TGGGTCCCAGGTCATCCG	5450	del	TGGGTCCCAGGTCATCCT	5490	A
3582A>G	GAATGTTGGAGGACCCA	F	5259	GAATGTTGGAGGACCCAA	5531	A	GAATGTTGGAGGACCCAG	5547	G
2539_2542  delAACT	ACAGCTGGATGAGCTGCT	F	5540	ACAGCTGGATGAGCTGCTA	5811	AACT	ACAGCTGGATGAGCTGCTG	5827	del
3790C>T	CCACTCTCACCCTGCATCT	F	5620	CCACTCTCACCCTGCATCTC	5867	C	CCACTCTCACCCTGCATCTT	5947	T
4115C>T	ACCTCCCTGCTGCAGCACTT	F	5989	ACCTCCCTGCTGCAGCACTTC	6236	C	ACCTCCCTGCTGCAGCACTTT	6316	T
4481G>A	GAATCTGACTGCCCAGATTG	F	6117	GAATCTGACTGCCCAGATTGA	6388	A	GAATCTGACTGCCCAGATTGG	6404	G
3828G>A	ACTCATCACCAACCTGTCATC	F	6270	ACTCATCACCAACCTGTCATCA	6541	A	ACTCATCACCAACCTGTCATCG	6557	G
3384A>C	CATGCTGGGGCTATCACCAGG	R	6447	CATGCTGGGGCTATCACCAGGG	6734	C	CATGCTGGGGCTATCACCAGGT	6774	A
2853A>C	GAGAACAGGTCAGCCACCACTA	R	6722	GAGAACAGGTCAGCCACCACTAG	7010	C	GAGAACAGGTCAGCCACCACTAT	7050	A
2988G>A	CATGTGCCCCCGCCTGTACCCTT	R	6888	CATGTGCCCCCGCCTGTACCCTTC	7135	G	CATGTGCCCCCGCCTGTACCCTTT	7215	A
3584G>A	GTCAGAATGTTGGAGGACCCAAC	F	7098	GTCAGAATGTTGGAGGACCCAACA	7369	A	GTCAGAATGTTGGAGGACCCAACG	7385	G
4401C>T	TAACTGACATCTGCTCAGCCTCAAC	R	7546	TAACTGACATCTGCTCAGCCTCAACA	7817	T	TAACTGACATCTGCTCAGCCTCAACG	7833	C
2587_2590  delGACT	ATCAGCTCAGCCCCCCCGAGACCTGA	F	7846	ATCAGCTCAGCCCCCCCGAGACCTGAC	8093	GACT	ATCAGCTCAGCCCCCCCGAGACCTGAG	8133	del
(5) 15-PLEX
3948T>G	TCTCAGCAGGTGCCTG	F	4873	TCTCAGCAGGTGCCTGG	5160	G	TCTCAGCAGGTGCCTT	5200	T
-1235A>G	GCACCACCCAGCCTAAT	R	5084	GCACCACCCAGCCTAATC	5332	G	GCACCACCCAGCCTAATT	5411	A
137_138insT	GAAGTCCACATGCAGCA	R	5188	GAAGTCCACATGCAGCAA	5460	T	GAAGTCCACATGCAGCAG	5476	—
4155C>T	CAGCCCCGGCCCAGCCAC	F	5376	CAGCCCCGGCCCAGCCACC	5623	C	CAGCCCCGGCCCAGCCACT	5703	T
82C>T	GTGTAGCGTGCAGCCCAGC	R	5830	GTGTAGCGTGCAGCCCAGCA	6101	T	GTGTAGCGTGCAGCCCAGCG	6117	C
-1584C>G	CTGGACAACTTGGAAGAACC	F	6135	CTGGACAACTTGGAAGAACCC	6382	C	CTGGACAACTTGGAAGAACCG	6422	G
2575C>A	GACCTGGGACCCAGCCCAGCC	F	6362	GACCTGGGACCCAGCCCAGCCC	6609	C	GACCTGGGACCCAGCCCAGCCA	6633	A
843T>G	ACTAGGACCTGTAGTCTGGGG	F	6502	ACTAGGACCTGTAGTCTGGGGG	6789	G	ACTAGGACCTGTAGTCTGGGGT	6829	T
-740C>T	ACAGACTCACACTGACACTTAG	R	6672	ACAGACTCACACTGACACTTAGA	6944	T	ACAGACTCACACTGACACTTAGG	6960	C
-678G>A	CTTTGTGTGGGTGATTTTCTGC	F	6769	CTTTGTGTGGGTGATTTTCTGCA	7041	A	CTTTGTGTGGGTGATTTTCTGCG	7057	G
-750_-749 delGA	TGTGACTGGTGTGTGTGAGAGA	F	6902	TGTGACTGGTGTGTGTGAGAGAA	7173	del	TGTGACTGGTGTGTGTGAGAGAG	7189	GA
2291G>A	CACTCGCCAAGTGCCAGCCTCCAC	R	7204	CACTCGCCAAGTGCCAGCCTCCACC	7451	G	CACTCGCCAAGTGCCAGCCTCCACT	7531	A
-1426C>T	GTGTGCCACCACGTCTAGCTTTTT	R	7286	GTGTGCCACCACGTCTAGCTTTTTA	7533	T	GTGTGCCACCACGTCTAGCTTTTTG	7573	C
1758G>T>A	TTGTGCCGCCTTCGCCAACCACTCC	F	7490	TTGTGCCGCCTTCGCCAACCACTCCA	7761	A	TTGTGCCGCCTTCGCCAACCACTCCG	7777	G
							TTGTGCCGCCTTCGCCAACCACTCCT	7817	T
-1000G>A	ACATCCTCCCGGGCTGCCTGAGGGT	R	7635	ACATCCTCCCGGGCTGCCTGAGGGTC	7882	G	ACATCCTCCCGGGCTGCCTGAGGGTT	7962	A

UD: Unextended mini–sequencing primer Direction; F: Forward UEP direction; R: Reverse UEP direction; UM: Unextended mini–sequencing primers Mass; ELM: Extended primer Lower Mass; EHM: Extended primer Higher Mass; ELC: Extended primer lower Mass nucleotide Called; EHC: Extended primer higher Mass nucleotide Called; All molecular weight masses are expressed in Dalton (Da).