Genotyping of CYP2D6 Polymorphisms by MALDI-TOF Mass Spectrometry in Sardinian People

Table 3

Sequences and molecular weights of unextended (UEP) and extended (EP) iPLEX primers.


	UEPs’ sequences	UD	UM	EP lower mass sequences	ELM	ELC	EP higher mass sequences	EHM	EHC

(1) 13-PLEX
3877G>A	CCTTCCGCTTCCACCCC	F		CCTTCCGCTTCCACCCCA		A	CCTTCCGCTTCCACCCCG		G
3183G>A	CCGCACCTGCCCTATCA	R		CCGCACCTGCCCTATCAC		G	CCGCACCTGCCCTATCAT		A
2615_2617 delAAG	GGCAGCCACTCTCACCT	R		GGCAGCCACTCTCACCTC		del	GGCAGCCACTCTCACCTT		AGA
2483G>T	GCGTAGGACCTTGCCAG	R		GCGTAGGACCTTGCCAGC		G	GCGTAGGACCTTGCCAGA		T
3853G>A	GAAGCGGAAGGGCTTCT	R		GAAGCGGAAGGGCTTCTC		G	GAAGCGGAAGGGCTTCTT		A
3277T>C	TGCAGCGCTTTGGGGACA	F		TGCAGCGCTTTGGGGACAC		C	TGCAGCGCTTTGGGGACAT		T
4180G>C	AGCTCATAGGGGGATGGG	R		AGCTCATAGGGGGATGGGC		G	AGCTCATAGGGGGATGGGG		C
2939G>A	TGGGCTCACGCTGCACATC	R		TGGGCTCACGCTGCACATCC		G	TGGGCTCACGCTGCACATCT		A
3288G>A	GATGTCATATGGGTCACAC	R		GATGTCATATGGGTCACACC		G	GATGTCATATGGGTCACACT		A
3176C>T	GGCCGTGTCCAACAGGAGAT	F		GGCCGTGTCCAACAGGAGATC		C	GGCCGTGTCCAACAGGAGATT		T
2850C>T	AGCTTCAATGATGAGAACCTG	F		AGCTTCAATGATGAGAACCTGC		C	AGCTTCAATGATGAGAACCTGT		T
2935A>C	AGTGCTCACGCTGCACATCCGGA	R		AGTGCTCACGCTGCACATCCGGAG		C	AGTGCTCACGCTGCACATCCGGAT		A
1757C>T	TTGTGCCGCCTTCGCCAACCACTC	F		TTGTGCCGCCTTCGCCAACCACTCC		C	TTGTGCCGCCTTCGCCAACCACTCT		T

(2) 14-PLEX
1661G>C	CAGAGGCGCTTCTCCGT	F		CAGAGGCGCTTCTCCGTC		C	CAGAGGCGCTTCTCCGTG		G
1869T>C	ACGGCTTTGTCCAAGAG	R		ACGGCTTTGTCCAAGAGA		T	ACGGCTTTGTCCAAGAGG		C
1846G>A	GGGGCGAAAGGGGCGTC	R		GGGGCGAAAGGGGCGTCC		G	GGGGCGAAAGGGGCGTCT		A
214G>C	TGGAGGGCGGCAGAGGT	F		TGGAGGGCGGCAGAGGTC		C	TGGAGGGCGGCAGAGGTG		G
19G>A	CTGGGGCTAGAAGCACTG	F		CTGGGGCTAGAAGCACTGA		A	CTGGGGCTAGAAGCACTGG		G
1707delT	GCGGCCTCCTCGGTCACCC	R		GCGGCCTCCTCGGTCACCCC		del	GCGGCCTCCTCGGTCACCCA		T
746C>G	GCGACCCAGCCTCCTGATC	R		GCGACCCAGCCTCCTGATCC		G	GCGACCCAGCCTCCTGATCG		C
997C>G	GAACAGGCGGGCGGTCGGC	R		GAACAGGCGGGCGGTCGGCC		G	GAACAGGCGGGCGGTCGGCG		C
100C>T	AATGCTGGGCTGCACGCTAC	F		AATGCTGGGCTGCACGCTACC		C	AATGCTGGGCTGCACGCTACT		T
1979T>C	GGACAGCCCGACTCCTCCTTC	R		GGACAGCCCGACTCCTCCTTCA		T	GGACAGCCCGACTCCTCCTTCG		C
-948C>A	CAGGCTGGGGCAAGGGCCTTC	F		CAGGCTGGGGCAAGGGCCTTCC		C	CAGGCTGGGGCAAGGGCCTTCA		A
1749A>G	GCCCATCACCCACCGGAGTGGT	R		GCCCATCACCCACCGGAGTGGTC		G	GCCCATCACCCACCGGAGTGGTT		A
77G>A	GTAGCGTGCAGCCCAGCGTTGG	R		GTAGCGTGCAGCCCAGCGTTGGC		G	GTAGCGTGCAGCCCAGCGTTGGT		A
1513C>T	AGCTGGACAGAGCCAGGGACTG	F		AGCTGGACAGAGCCAGGGACTGC		C	AGCTGGACAGAGCCAGGGACTGT		T

(3) 13-PLEX
984A>G	GCGAGTGTCCTCGCCG	R		GCGAGTGTCCTCGCCGC		G	GCGAGTGTCCTCGCCGT		A
1863_1864ins TTTCGCCCC	GACGCCCCTTTCGCCCC	F		GACGCCCCTTTCGCCCCA		–	GACGCCCCTTTCGCCCCT		TTTCGCCCC
1039C>T	ACCCAGATCCTGGGTTT	F		ACCCAGATCCTGGGTTTC		C	ACCCAGATCCTGGGTTTT		T
1943G>A	CCAGCAGCCTGAGGAAG	R		CCAGCAGCCTGAGGAAGC		G	CCAGCAGCCTGAGGAAGT		A
974C>A	CGGCCGTGCGCGAGGCG	F		CGGCCGTGCGCGAGGCGC		C	CGGCCGTGCGCGAGGCGA		A
1704C>G	GGCAAGAAGTCGCTGGAG	F		GGCAAGAAGTCGCTGGAGC		C	GGCAAGAAGTCGCTGGAGG		G
310G>T	TAAATGCCCTTCTCCAGGA	R		TAAATGCCCTTCTCCAGGAC		G	TAAATGCCCTTCTCCAGGAA		T
957C>T	GTCGTGCTCAATGGGCTGG	F		GTCGTGCTCAATGGGCTGGC		C	GTCGTGCTCAATGGGCTGGT		T
883G>C	TCCCCGAAGCGGCGCCGCAA	R		TCCCCGAAGCGGCGCCGCAAC		G	TCCCCGAAGCGGCGCCGCAAG		C
1659G>A	CGCGAGCAGAGGCGCTTCTCC	F		CGCGAGCAGAGGCGCTTCTCCA		A	CGCGAGCAGAGGCGCTTCTCCG		G
31G>A	AGCAGGAAGATGGCCACTATCA	R		AGCAGGAAGATGGCCACTATCAC		G	AGCAGGAAGATGGCCACTATCAT		A
1724C>T	GCGAAGGCGGCACAAAGGCAGGC	R		GCGAAGGCGGCACAAAGGCAGGCA		T	GCGAAGGCGGCACAAAGGCAGGCG		C
124G>A	TCACATGCAGCAGGTTGCCCAGCC	R		TCACATGCAGCAGGTTGCCCAGCCC		G	TCACATGCAGCAGGTTGCCCAGCCT		A

(4) 14-PLEX
3198C>G	ACCCATCTCTGGTCGCC	R		ACCCATCTCTGGTCGCCC		G	ACCCATCTCTGGTCGCCG		C
2549delA	TGGGTCCCAGGTCATCC	R		TGGGTCCCAGGTCATCCG		del	TGGGTCCCAGGTCATCCT		A
3582A>G	GAATGTTGGAGGACCCA	F		GAATGTTGGAGGACCCAA		A	GAATGTTGGAGGACCCAG		G
2539_2542 delAACT	ACAGCTGGATGAGCTGCT	F		ACAGCTGGATGAGCTGCTA		AACT	ACAGCTGGATGAGCTGCTG		del
3790C>T	CCACTCTCACCCTGCATCT	F		CCACTCTCACCCTGCATCTC		C	CCACTCTCACCCTGCATCTT		T
4115C>T	ACCTCCCTGCTGCAGCACTT	F		ACCTCCCTGCTGCAGCACTTC		C	ACCTCCCTGCTGCAGCACTTT		T
4481G>A	GAATCTGACTGCCCAGATTG	F		GAATCTGACTGCCCAGATTGA		A	GAATCTGACTGCCCAGATTGG		G
3828G>A	ACTCATCACCAACCTGTCATC	F		ACTCATCACCAACCTGTCATCA		A	ACTCATCACCAACCTGTCATCG		G
3384A>C	CATGCTGGGGCTATCACCAGG	R		CATGCTGGGGCTATCACCAGGG		C	CATGCTGGGGCTATCACCAGGT		A
2853A>C	GAGAACAGGTCAGCCACCACTA	R		GAGAACAGGTCAGCCACCACTAG		C	GAGAACAGGTCAGCCACCACTAT		A
2988G>A	CATGTGCCCCCGCCTGTACCCTT	R		CATGTGCCCCCGCCTGTACCCTTC		G	CATGTGCCCCCGCCTGTACCCTTT		A
3584G>A	GTCAGAATGTTGGAGGACCCAAC	F		GTCAGAATGTTGGAGGACCCAACA		A	GTCAGAATGTTGGAGGACCCAACG		G
4401C>T	TAACTGACATCTGCTCAGCCTCAAC	R		TAACTGACATCTGCTCAGCCTCAACA		T	TAACTGACATCTGCTCAGCCTCAACG		C
2587_2590 delGACT	ATCAGCTCAGCCCCCCCGAGACCTGA	F		ATCAGCTCAGCCCCCCCGAGACCTGAC		GACT	ATCAGCTCAGCCCCCCCGAGACCTGAG		del

(5) 15-PLEX
3948T>G	TCTCAGCAGGTGCCTG	F		TCTCAGCAGGTGCCTGG		G	TCTCAGCAGGTGCCTT		T
-1235A>G	GCACCACCCAGCCTAAT	R		GCACCACCCAGCCTAATC		G	GCACCACCCAGCCTAATT		A
137_138insT	GAAGTCCACATGCAGCA	R		GAAGTCCACATGCAGCAA		T	GAAGTCCACATGCAGCAG		—
4155C>T	CAGCCCCGGCCCAGCCAC	F		CAGCCCCGGCCCAGCCACC		C	CAGCCCCGGCCCAGCCACT		T
82C>T	GTGTAGCGTGCAGCCCAGC	R		GTGTAGCGTGCAGCCCAGCA		T	GTGTAGCGTGCAGCCCAGCG		C
-1584C>G	CTGGACAACTTGGAAGAACC	F		CTGGACAACTTGGAAGAACCC		C	CTGGACAACTTGGAAGAACCG		G
2575C>A	GACCTGGGACCCAGCCCAGCC	F		GACCTGGGACCCAGCCCAGCCC		C	GACCTGGGACCCAGCCCAGCCA		A
843T>G	ACTAGGACCTGTAGTCTGGGG	F		ACTAGGACCTGTAGTCTGGGGG		G	ACTAGGACCTGTAGTCTGGGGT		T
-740C>T	ACAGACTCACACTGACACTTAG	R		ACAGACTCACACTGACACTTAGA		T	ACAGACTCACACTGACACTTAGG		C
-678G>A	CTTTGTGTGGGTGATTTTCTGC	F		CTTTGTGTGGGTGATTTTCTGCA		A	CTTTGTGTGGGTGATTTTCTGCG		G
-750_-749 delGA	TGTGACTGGTGTGTGTGAGAGA	F		TGTGACTGGTGTGTGTGAGAGAA		del	TGTGACTGGTGTGTGTGAGAGAG		GA
2291G>A	CACTCGCCAAGTGCCAGCCTCCAC	R		CACTCGCCAAGTGCCAGCCTCCACC		G	CACTCGCCAAGTGCCAGCCTCCACT		A
-1426C>T	GTGTGCCACCACGTCTAGCTTTTT	R		GTGTGCCACCACGTCTAGCTTTTTA		T	GTGTGCCACCACGTCTAGCTTTTTG		C
1758G>T>A	TTGTGCCGCCTTCGCCAACCACTCC	F		TTGTGCCGCCTTCGCCAACCACTCCA		A	TTGTGCCGCCTTCGCCAACCACTCCG		G
1758G>T>A	TTGTGCCGCCTTCGCCAACCACTCC	F		TTGTGCCGCCTTCGCCAACCACTCCA		A	TTGTGCCGCCTTCGCCAACCACTCCT		T
-1000G>A	ACATCCTCCCGGGCTGCCTGAGGGT	R		ACATCCTCCCGGGCTGCCTGAGGGTC		G	ACATCCTCCCGGGCTGCCTGAGGGTT		A

UD: Unextended mini–sequencing primer Direction; F: Forward UEP direction; R: Reverse UEP direction; UM: Unextended mini–sequencing primers Mass; ELM: Extended primer Lower Mass; EHM: Extended primer Higher Mass; ELC: Extended primer lower Mass nucleotide Called; EHC: Extended primer higher Mass nucleotide Called; All molecular weight masses are expressed in Dalton (Da).

International Scholarly Research Notices

Genotyping of CYP2D6 Polymorphisms by MALDI-TOF Mass Spectrometry in Sardinian People

Table 3