Interaction of Normal and Sickle Hemoglobins for Sodium Dodecylsulphate and Hydrogen Peroxide at pH 5.0 and 7.2

Table 1

Interaction parameters of Hb with SDS and  H₂O₂ at pH’s 5.0 and 7.2.

Hemoglobin sample
Treatment	Parameters	HbA	HbAS	HbS
SDS, pH 5.0	Δ𝐴max	1.000 ± 0.005577	1.000 ± 0.006371	1.000 ± 0.05466
	ℎ (𝑛)	4.081 ± 2.382	4.954 ± 2.025	6.050 ± 4.771
	𝐾𝑑 (mM)	0.05641 ± 0.006	0.07368 ± 0.0097	0.05977 ± 0.0072
	𝑅^2	0.9536	0.9479	0.9567
SDS, pH 7.2	Δ𝐴max	0.9963 ± 0.044	0.9951 ± 0.1448	0.9945 ± 0.09589
	ℎ (𝑛)	2.232 ± 0.2384	1.481 ± 0.2750	1.824 ± 0.3063
	𝐾𝑑 (mM)	0.2325 ± 0.016	0.2873 ± 0.07246	0.2547 ± 0.03908
	𝑅^2	0.9974	0.9929	0.9938
H2O2, pH 5.0	𝐴max	0.9959 ± 0.06882	0.9968 ± 0.05220	0.9965 ± 0.08263
	ℎ (𝑛)	1.552 ± 0.2906	1.558 ± 0.3135	1.428 ± 0.3145
	𝐾𝑑 (mM)	0.1329 ± 0.01874	0.08883 ± 0.01005	0.1242 ± 0.02161
	𝑅^2	0.9915	0.9909	0.9893
H2O2, pH 7.2	𝐴max	0.9954 ± 0.2002	0.9946 ± 0.08353	0.9963 ± 0.3365
	ℎ (𝑛)	1.123 ± 0.1706	1.570 ± 0.2138	1.121 ± 0.2203
	𝐾𝑑 (mM)	0.4310 ± 0.1681	0.2364 ± 0.03510	0.5772 ± 0.3536
	𝑅^2	0.9963	0.9959	0.9941