Table of Contents
ISRN Virology
Volume 2013 (2013), Article ID 751904, 9 pages
http://dx.doi.org/10.5402/2013/751904
Research Article

Determination of Infectious Bovine Viral Diarrhea Virus in Bovine Lung Lavages by a Combination of Virus Propagation in Cell Culture and Quantitative Real-Time PCR

Labor Dr. Merk & Kollegen GmbH, Beim Braunland 1, 88416 Ochsenhausen, Germany

Received 15 May 2013; Accepted 4 June 2013

Academic Editors: A. Doglio, A. Kfutwah, B. Kim, A. Mastino, and C. Risco

Copyright © 2013 Benjamin Zeitler and Ingrid Rapp. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Raw data (cycle thresholds) of the BVDV copy number determination by qRT-PCR: unprocessed bovine lung lavages (Table S1)

Raw data (cycle thresholds) of the qRT-PCR calibration curve: BVDV spiked in bovine lung lavage (Table S2)

Raw data (cycle thresholds) of the qRT-PCR calibration curve: BVDV spiked in ddH2O (Table S3)

BVDV propagation kinetics in MDBK cells: Raw data (cycle thresholds) of the BVDV copy number determination by qRT-PCR after infection of cells with defined amounts of virus (Table S4)

BVDV propagation kinetics in MDBK cells: Raw data (cycle thresholds) of the BVDV copy number determination by qRT-PCR after subcultivation of lung lavage inoculated cell cultures (Table S5)

Raw data (cycle thresholds) of calibrator samples

IFT: BVDV positive and negative control samples

  1. Supplementary Materials