Proteomics Characterization of the Secretome from Rat Pancreatic Stellate Cells with ATP-Binding Cassette Transporters (ABCG2) and NCAM Phenotype
PaSCs-ABCG2(+) secrete TGFβ1, which causes the inhibition of proliferation but has no effect on PDX1 increase. (a) Quantification of active TGFβ1 in supernatant of PaSC cultured (conditioned media) for 24 hours. Active TGFβ1 was measured directly by ELISA, compared to TGFβ1 in the basal media (). (b) Expression of TGFβ receptors in ARIP cells detected by RT-PCR. Lanes: 1-basal media, 2-conditioned media, 3-(–)RT. (c) Proliferation rate of ARIP cells (BrdU assay) after different stimulations: basal media, basal media plus nonimmune IgG 20 μg/mL, conditioned media, conditioned media plus Pan TGFβ 20 μg/mL, basal media plus TGFβ1 10 ng/mL, or basal media plus pan TGFβ and TGFβ1. Results are expressed as percentage of control values (basal media) and are representative of 3 separate experiments. The neutralising antibody can revert the inhibition of proliferation in ARIP cells treated with conditioned media. * indicate statistical significance with . (d) PDX1 expression of ARIP cells treated with basal media, conditioned media, conditioned media plus pan TGFβ 20 μg/mL, basal media plus TGFβ1 10 ng/mL, or basal media plus pan TGFβ and TGFβ1. Results are expressed as fold increase of control values (basal media) and are representative of 6 independent experiments. NS indicates no statistical significance.