Table of Contents
Journal of Allergy
Volume 2012, Article ID 580873, 9 pages
http://dx.doi.org/10.1155/2012/580873
Research Article

Comparison of CD63 Upregulation Induced by NSAIDs on Basophils and Monocytes in Patients with NSAID Hypersensitivity

1Service d'Hématologie et d'Immunologie Biologique, Hôpital Tenon (AP-HP), Université Pierre et Marie Curie, 4 Rue de la Chine, 75020 Paris, France
2Service de Dermatologie et Allergologie, Hôpital Tenon (AP-HP), Université Pierre et Marie Curie, 4 Rue de la Chine, 75020 Paris, France

Received 28 June 2011; Accepted 11 September 2011

Academic Editor: Hae-Sim Park

Copyright © 2012 N. Abuaf et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. An in vitro basophil activation test, based on the detection of CD63 upregulation induced by NSAIDs, has been described. Its clinical significance remains controversial. Objectives. In patients with a history of nonallergic NSAID hypersensitivity, stratified according to the severity of the symptoms, to assess with NSAIDs the predictive value of basophil (BAT) and monocyte (MAT) activation tests. Patients/Methods. Sixty patients who had NSAIDs-induced or exacerbated urticaria/angiooedema and 20 controls was included. After incubation with NSAIDs or acetaminophen, leukocytes were analysed for CD63 upregulation. Results. With aspirin, the sensitivity (37%) and specificity (90%) of BAT agree with already published results. In contrast, when patients had had cutaneous and visceral reactions, the frequency of positive BAT 14/22 (64%, P<0.001) or MAT 10/22 (46%, P<0.01) were increased. Conclusions. Positive tests were more frequent among patients having a severe hypersensitivity contrasting with the other patients who had results similar to controls.