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Figure 4: Effects of feeding diets differing in sulfur amino acid levels on the 4EBP1 phosphorylation status in liver of rats. Values are means ± SEM for 4 rats. Values represented by bars not labeled with the same letter are significantly different at by ANOVA and Tukey’s comparison; values for γ4EBP1/total 4EBP1 were transformed to square roots prior to statistical analysis. A representative western blot is shown; equal amounts of total soluble protein were loaded per lane with the order of samples, being the same as for the bar graphs. A higher percentage of polyacrylamide and a longer run time were used for electrophoresis to obtain better separation of the bands than for the western blots shown in Figure 3. The amounts of α4EBP1, β4EBP1 and γ4EBP1 were normalized by actin. The ratio of γ4EBP1 to total 4EBP1 is the ratio of the density of the γ4EBP1 band to the sum of the densities for all three 4EBP1 bands.