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Journal of Applied Mathematics
Volume 2013, Article ID 378253, 13 pages
http://dx.doi.org/10.1155/2013/378253
Research Article

Design and Simulation of a Fused Silica Space Cell Culture and Observation Cavity with Microfluidic and Temperature Controlling

Shangchun Fan,1,2,3 Jinhao Sun,1,2,3 Weiwei Xing,1,2,3 Cheng Li,1,2,3 and Dongxue Wang1,2,3

1School of Instrument Science and Opto-Electronics Engineering, Beihang University, 37 Xueyuan Road, Haidian District, Beijing 100191, China
2Key Laboratory of Precision Opto-Mechatronics Technology, Ministry of Education, Beihang University, Beijing 100191, China
3Key Laboratory of Inertial Science and Technology for National Defence, Beihang University, Beijing 100191, China

Received 11 June 2013; Accepted 27 September 2013

Academic Editor: Bo Yu

Copyright © 2013 Shangchun Fan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We report a principle prototype of space animal cell perfusion culture and observation. Unlike previous work, our cell culture system cannot only realize microfluidic and temperature controlling, automatic observation, and recording but also meet an increasing cell culture at large scale operation and overcome shear force for animal cells. A key component in the system is ingenious structural fused silica cell culture cavity with the wedge-shaped connection. Finite volume method (FVM) is applied to calculate its multipoint flow field, pressure field, axial velocity, tangential velocity, and radial velocity. In order to provide appropriate flow rate, temperature, and shear force for space animal cell culture, a closed-loop microfluidic circuit and proportional, integrating, and differentiation (PID) algorithm are employed. This paper also illustrates system architecture and operating method of the principle prototype. The dynamic culture, autofocus observation, and recording of M763 cells are performed successfully within 72 h in the laboratory environment. This research can provide a reference for space flight mission that carries an apparatus with similar functions.