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Journal of Automatic Chemistry
Volume 11, Issue 2, Pages 80-83

An evaluation of osmolality measurement by freezing point depression using micro-amounts of sample

Biochemistry Department, Royal North Shore Hospital of Sydney, Sydney 2065, Australia

Copyright © 1989 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


An evaluation of the Advanced micro-osmometer is presented. This instrument has been shown to have an excellent analytical precision (within-run CV = 0.59%, between-day CV = 0.58%). It is accurate over an analytical range of 0-2000 mmol/kg of osmolality shown by linearity studies and split sample correlations against vapour pressure osmometry, freezing point osmometry and an external quality assurance programme. Analytical errors due to operator technique are almost eliminated because of good instrument design. Preliminary results on whole-blood osmolality are included. The required sample size of 20 μl permits osmolality measurements on most clinical samples. It is concluded that the Advanced micro-osmometer satisfies laboratory requirements.