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Journal of Automatic Chemistry
Volume 11, Issue 6, Pages 280-283

Automated HPLC monitoring of broth components on bioreactors

EPFL, IGE-Laboratoire de Génie Biologique, Lausanne CH-1015, Switzerland

Copyright © 1989 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Under proper operating conditions, a low dead volume continuous filtration module operated on biological broths (yeast and bacteria suspensions in stirred reactors) still fulfills the flow-rate requirements of an analytical apparatus (for example HPLC or FIA) without membrane regeneration. The filtrate stream has been successfully connected to a bioreactor in order to perform the automated HPLC analysis of broth components. The monitoring of the carbon source (lactose), and minor products (glycerol, acetate and succinate) during a yeast culture (Kluyveromyces marxianus) is shown.