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Journal of Automatic Chemistry
Volume 14, Issue 4, Pages 145-149

Plasma amino-acid determinations by reversed-phase HPLC: Improvement of the orthophthalaldehyde method and comparison with ion exchange chromatography

1Laboratoire de Biochimie, UER des Sciences, Pharmaceutiques et Biologiques, Université Paris XI, Chatenay, 92296, France
2Laboratoire de Biochimie, UER des Sciences Pharmaceutiques et Biologiques, 5 rue J. B. Clément, Chatenay-Malabry Cedex 92296, France

Copyright © 1992 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


RP-HPLC with automated pre-column OPA derivatization is clearly a suitable alternative for assaying physiological AA and may be particularly useful for AA present at low concentrations (free tryptophan, plasma 3-methylhistidine).