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Journal of Automatic Chemistry
Volume 17, Issue 2, Pages 73-76

Evaluation of an automatic HPLC analyser for thalassemia and haemoglobin variants screening

1Istituto di Clinica e Biologia dell'Età Evolutiva, Università degli Studi di Cagliari, Via Jenner s/n, Cagliari 09100, Italy
2Dipartimento Scienze e Tecnologie Biomediche, Univcrsità degli Studi di Milano, Via Olgettina, 60, Milan 20132, Italy
3Istituto di Clinica e Biologia dell'Età Evolutiva, Ospedale Regionale Microcitemie USL 21, Università degli Studi di Cagliari, Via Jenner s/n, Cagliari 09100, Italy

Copyright © 1995 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In this paper the authors report the evolution of a new automatic HPLC analyser for screening haemoglobinopathies. HbA2 and F determinations are accurate and reproducible. The analysis time is short (6.5 min) and there is a good separation between the HbA2 values of β-thalassemia carriers from normals and α-thalassemia carriers, with no overlap between these groups. In addition, the system is also able to detect and quantitate most of the haemoglobin variants, particularly those (HbS, HbC, HbE and Hb Lepore) able to interact with β-thalassemia and could make haemoglobin electrophoresis unnecessary in all samples. The ease of operation and the limited technical work make this system especially suitable for laboratories with a high workload and allow the cost of screening to be reduced.