S. Kitahashi, N. Tatsumi, S. Tagawa, T. Matsui, M. Higashihata, H. Shintaku, S. Tomoda, I. Tsuda, "Diagnosis of infections in newborns using a new particle-mediated immunoassay for serum C-reactive protein", Journal of Analytical Methods in Chemistry, vol. 20, Article ID 140671, 4 pages, 1998. https://doi.org/10.1155/S146392469800025X
Diagnosis of infections in newborns using a new particle-mediated immunoassay for serum C-reactive protein
C-reactive protein (CRP) levels were measured using a new particle-mediated immunoassay. Tests for precision and linearity of this method gave satisfactory results. The minimum sensitivity of the assay was 1 ng/ml. Interference by bilirubin (<220mg/l) and haemoglobin (<20g/l) was not observed. Using this method, CRP was assayed as a means of monitoring for infection in newborns up to 72 h after delivery. The pattern of time course elevation curves was similar for both groups (10 healthy subjects and 26 patients), but the serum CRP (ng/ml) of infected newborns rose significantly higher than in healthy subjects at 24 h after birth. The rate of increase of CRP (∆CRP; ng/ml/h) may be a more useful parameter to detect infection, since a significant change in ∆CRP was apparent only 12 h after birth. The reported method was reliable and the parameters obtained were considered clinically useful for early detection of infection.
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